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4. Use your knowledge of intermolecular interactions to predict which of these substance will run further...
2. What are the phases involved in TLC? 3. What is coated on the TLC plate?...
2. What are the phases involved in TLC? 3. What is coated on the TLC plate? 4. What is the purpose of lining the wall of the TLC chamber with paper? 5. Why is it important to use a small amount of the mobile phase? 6. Why should you remove the TLC plate before the mobile phase reaches the top? 7. What type of compounds are visible with UV light? 8. With a polar stationary phase and non-polar mobile phase,...
1. What is the purpose of Thin-Layer Chromatography (TLC)? 2. What are the phases involved in...
1. What is the purpose of Thin-Layer Chromatography (TLC)? 2. What are the phases involved in TLC? 3. What is coated on the TLC plate? 4. What is the purpose of lining the wall of the TLC chamber with paper? 5. Why is it important to use a small amount of the mobile phase? 6. Why should you remove the TLC plate before the mobile phase reaches the top? 7. What type of compounds are visible with UV light? 8....
slightly confused I thought thought Rf 0 was solid andRf1 is slightly liquid please any...
slightly confused I thought Rf 0 was solid and
Rf1 is slightly liquid please any help is greatly appreciated.3. Two extreme values for the Retention Factor are 0 and 1 a) Explain what each Re value means in terms of the substance's affinity for the mobile phase and for the stationary phase. b) Describe the features of molecules that would have these extreme Rr values under the conditions in this experiment: the solvent an 80:20 mix of petroleum ether and acetone, the...
Why would it be unwise to use 100% ethyl acetate as the first mobile phase you...
Why would it be unwise to use 100% ethyl acetate as the first mobile phase you test when determining the best mobile phase to separate the mixture of analgesics in Procedure? Why do polar compounds move slower up the silica TLC plates than nonpolar compounds? Why does increasing the polarity of the mobile phase increase the rate at which all compounds move up the plate?
1) Calculate the Rf value of a spot that travels 5.7 cm, with a solvent front...
1) Calculate the Rf value of a spot that travels 5.7 cm, with a solvent front that travels 13 cm. 2) A student spots an unknown sample on a TLC plate and develops it in pentane solvent. Only one spot, for which the Rf value is 0.05, is observed. Is the unknown material a pure compound? What can be done to verify the purity of the sample using thin-layer chromatography? 3) You try to synthesize n-hexyl bromide as a product...
can you please answer all 1. When you run a TLC of the reaction in ethyl...
can
you please answer all
1. When you run a TLC of the reaction in ethyl acetate : hexanes 1:1, the plate below is produced. Give a solvent system that would be expected to produce greater separation and a BRIEF (8 words) justification about your choice. (5 points) 7 6 5 4 3 b. Calculate the R for the bottom spot. (5 points) c. Which compound on the TLC plate is the most polar? (5 points) 2. Why is it...
A student monitored the reaction progress of the reduction of 4-Nitrobenzaldehyde to 4-Nitrobenzyl alcohol using TLC....
A student monitored the reaction progress of the reduction of
4-Nitrobenzaldehyde to 4-Nitrobenzyl alcohol using TLC. 30% ethyl
acetate in petroleum ether was used as the elution solvent. TLC
plates available in the laboratory were coated with the common
absorbent silica gel (SiO2.xH2O) which
contains polar hydroxyl (OH-) groups.
c. The crude product moves slower
than the starting material with the mobile
phase in the TLC plate. Briefly
explain a possible reason for the
observation based on the chemical
structures...
Background In this lab, we are going to learn how to use a powerful separation technique...
Background In this lab, we are going to learn how to use a powerful separation technique called chromatography which can separate compounds that are quite similar to one another. Chromatography is a general type of separation in which a mixture of compounds passes through a stationary phase. Different compounds have different relative affinities for the mixture traveling along the stationary phase, or the mobile phase, and the stationary phase, causing these compounds to separate from one another. More specifically, we’re...
Classify and breifky explain the different patterns of genetic inheritance in humans Questions for the Thin...
Classify and breifky explain the different patterns of genetic
inheritance in humans
Questions for the Thin Layer Chromatography Laboratory 1. Why can't you use a pen to mark the origin of a TLC plate? Be explicit in your answer. 2. You were impatient to see how long it would take for your sample to resolve on the TLC plate, so you constantly removed the top from the chamber. Please explain why this is an incorrect practice. 3. What happens when...
Question 1 Which of the following is the advantage of straight packed column? Selected Answer: It...
Question 1 Which of the following is the advantage of straight packed column? Selected Answer: It can be repacked easily Answers It can be packed uniformly It can be repacked easily It is compact It is easier to heat it evenly Question 2 Which of the following statements most accurately describes thin-layer chromatography? Selected Answer: technique used to separate non-volatile mixtures, the mobile phase has different properties from the stationary phase. Answers The mobile phase is the solvent also called...
2. What are the phases involved in TLC? 3. What is coated on the TLC plate? 4. What is the purpose of lining the wall of the TLC chamber with paper? 5. Why is it important to use a small amount of the mobile phase? 6. Why should you remove the TLC plate before the mobile phase reaches the top? 7. What type of compounds are visible with UV light? 8. With a polar stationary phase and non-polar mobile phase,...
1. What is the purpose of Thin-Layer Chromatography (TLC)? 2. What are the phases involved in TLC? 3. What is coated on the TLC plate? 4. What is the purpose of lining the wall of the TLC chamber with paper? 5. Why is it important to use a small amount of the mobile phase? 6. Why should you remove the TLC plate before the mobile phase reaches the top? 7. What type of compounds are visible with UV light? 8....
slightly confused I thought Rf 0 was solid and
Rf1 is slightly liquid please any help is greatly appreciated.3. Two extreme values for the Retention Factor are 0 and 1 a) Explain what each Re value means in terms of the substance's affinity for the mobile phase and for the stationary phase. b) Describe the features of molecules that would have these extreme Rr values under the conditions in this experiment: the solvent an 80:20 mix of petroleum ether and acetone, the...
Why would it be unwise to use 100% ethyl acetate as the first mobile phase you test when determining the best mobile phase to separate the mixture of analgesics in Procedure? Why do polar compounds move slower up the silica TLC plates than nonpolar compounds? Why does increasing the polarity of the mobile phase increase the rate at which all compounds move up the plate?
1) Calculate the Rf value of a spot that travels 5.7 cm, with a solvent front that travels 13 cm. 2) A student spots an unknown sample on a TLC plate and develops it in pentane solvent. Only one spot, for which the Rf value is 0.05, is observed. Is the unknown material a pure compound? What can be done to verify the purity of the sample using thin-layer chromatography? 3) You try to synthesize n-hexyl bromide as a product...
can
you please answer all
1. When you run a TLC of the reaction in ethyl acetate : hexanes 1:1, the plate below is produced. Give a solvent system that would be expected to produce greater separation and a BRIEF (8 words) justification about your choice. (5 points) 7 6 5 4 3 b. Calculate the R for the bottom spot. (5 points) c. Which compound on the TLC plate is the most polar? (5 points) 2. Why is it...
A student monitored the reaction progress of the reduction of
4-Nitrobenzaldehyde to 4-Nitrobenzyl alcohol using TLC. 30% ethyl
acetate in petroleum ether was used as the elution solvent. TLC
plates available in the laboratory were coated with the common
absorbent silica gel (SiO2.xH2O) which
contains polar hydroxyl (OH-) groups.
c. The crude product moves slower
than the starting material with the mobile
phase in the TLC plate. Briefly
explain a possible reason for the
observation based on the chemical
structures...
Classify and breifky explain the different patterns of genetic
inheritance in humans
Questions for the Thin Layer Chromatography Laboratory 1. Why can't you use a pen to mark the origin of a TLC plate? Be explicit in your answer. 2. You were impatient to see how long it would take for your sample to resolve on the TLC plate, so you constantly removed the top from the chamber. Please explain why this is an incorrect practice. 3. What happens when...
Question 1 Which of the following is the advantage of straight packed column? Selected Answer: It can be repacked easily Answers It can be packed uniformly It can be repacked easily It is compact It is easier to heat it evenly Question 2 Which of the following statements most accurately describes thin-layer chromatography? Selected Answer: technique used to separate non-volatile mixtures, the mobile phase has different properties from the stationary phase. Answers The mobile phase is the solvent also called...
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