Question

1. What is the purpose of Thin-Layer Chromatography (TLC)? 2. What are the phases involved in TLC? 3. What is coated on the TLC plate? 4. What is the purpose of lining the wall of the TLC chamber with paper? 5. Why is it important to use a small amount of the mobile phase? 6. Why should you remove the TLC plate before the mobile phase reaches the top? 7. What type of compounds are visible with UV light? 8. With a polar stationary phase and non-polar mobile phase, rank the following compounds in order of lowest Rivalue to highest. 9. Why should you not use a pen to draw on your TLC plate?

Answer 1

purpose of thin-layer chromatography ELC). = Thin layer chromatography (TLC) is a technique a used for the separation of mixtures of chemicals compounds on a thin layer of adsorbent material. It is one of the most widely used technique a for the separation and final purification of organic compounds.

" The can also be used in quantitative analysis. main purpose is for the identife- cation of compounds like acids, alcoho - proteins, alkaloids , amines , antibiotics, 1 ek. 2 - It also checks the performance of 3 other separation processes. by The evaluate reaction process by assessment a of intermediates i reaction course, etc.