Problem 1
Thin layer Chromatography consist of three parts:
The analyte, the stationary phase and mobile phase.
Match each of these terms to what is was in out experiment.
Stationary Phase ____ a) The solvent
Mobile Phase ____ b) Silica
Analyte ____ c) One of the analgesiscs
Problem 2
Complete the sentences
Analytes on a TLC should have a(n) _________ Rf in a less polar solvent.
Analytes on a TLC should have a(n) _________ Rf in a more polar solvent.
a) Equal
b) Larger
c) Smaller
Problem 1 Thin layer Chromatography consist of three parts: The analyte, the stationary phase and mobile...
Thin Layer Chromatography (TLC) is a common technique that is routinely used in chemistry labs. It is simple, inexpensive, and very useful. Select the TRUE statement. More polar compounds travel faster on a TLC plate than less polar compounds. If compound A has a smaller Rf value than compound B, we can conclude that compound A is more polar than B. The stationary phase of a TLC silica gel plate is nonpolar. Rr is calculated as the distance traveled...
In Thin Layer Chromatography Experiments, would a more polar compound give a smaller or larger Rf value then a less polar compound? Explain your answer by referring to TLC theory (stationary phase, moving phase, polarity). Thanks!
In thin layer chromatography, would a more polar solvent increase or decrease the Rf values. Explain your answer by referring to TLC theory (stationary phase, moving phase, polarity...)
Please draw a thin chromatography plate with the following terms labelled 1. Origin 2. Stationary Phase 3. Mobile Phase 4. Solvent Front 5. Component "spot" 6. Rf value 7. Development 8. Visualization of spots Define questions 9 and 10 9. What is meant by differential partitioning between stationary and mobile phase? 10. What is the recommended procedure for cleaning a TLC spotting capillary?
2. An unknown compound was analyzed using thin layer chromatography, its constituted of two components (A and B) as shown in the TLC (Silica coated stationary phase; Chloroform and Ethyl acetate as mobile phase). The mixture was then loaded onto column with silica as the stationary phase and the same solvent system was used. (2 points) Column Chromatography TLC time A В Predict the order of elution of the compounds (A and B). which one would be eluted first/last and...
Which of the following statements regarding thin layer chromatography are TRUE? Partition chromatography separates compounds based on how they distribute themselves between the stationary and mobile phases. In thin layer chromatography, a polar organic compound will be attracted to the adsorbent and therefore travel further up the plate than a non-polar compound The Rf value is independent of the adsorbent material used. In thin layer chromatography, a polar organic compound will be repelled by the adsorbent and therefore travel not...
1. which of the following are not absorbent for TLC? a. magnesium silicate b. polyamides c. silica gel-F ( fluorescing indicator added) d. starch e. alumina f. none of the above 2. select the best answer from the statements below about thin layer chromatography A. in normal phase thin layer chromatography the stationary phase is polar silica gel b. in normal phase thin layer chromatography the mobile phase is an organic solvent or a mixture of organic solvents Which is...
1. What is the purpose of Thin-Layer Chromatography (TLC)? 2. What are the phases involved in TLC? 3. What is coated on the TLC plate? 4. What is the purpose of lining the wall of the TLC chamber with paper? 5. Why is it important to use a small amount of the mobile phase? 6. Why should you remove the TLC plate before the mobile phase reaches the top? 7. What type of compounds are visible with UV light? 8....
1. List two errors in the typical student set up for performing thin layer chromatography illustrated here. 2. Define the following terms: Analyte: Stationary Phase: Mobile phase: Solvent Front: Origin: Rf Value: 3. Compound y traveled 6.2 cm while the solvent front traveled 11.1 cm. Calculate the Rf value for compound y. 4. Compound Z has an Rf value of 0725. When a chromatogram was developed, it moved 3.3 cm from the origin. How many cm did the solvent front...
Can you help me answer the questions for the Introduction/Background? 1. Introduced the techniques of thin-layer and flask column chromatography. Discuss how a solvent is elected as eluent. 2. Discuss all the applications of TLC. What is it used for? 3. Discuss the mobile and stationary phases of TLC and column chromatography. 4. Explain how column chromatography can be used to separate a mixture on a larger scale than TLC. Post-Lab: Objective State the objective of the experiment in your...