In thin layer chromatography, would a more polar solvent increase or decrease the Rf values. Explain...
In thin layer chromatography, would a more polar solvent increase or decrease the Rf values. Explain your answer by referring to TLC theory (stationary phase, moving phase, polarity...)
Homework Answers
Usually, the stationary phase is more polar compared to mobile phase.
If the polarity of mobile phase increases, then the eluting power of the solvent also decreases. This will take the sample to lesser distance and thus Rf value will be low.
If the mobile phase is non polar, then the solvent travels to greater extent and thus Rf value will be high.
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In thin layer chromatography, would a more polar solvent increase or decrease the Rf values. Explain...
In Thin Layer Chromatography Experiments, would a more polar compound give a smaller or larger Rf...
In Thin Layer Chromatography Experiments, would a more polar compound give a smaller or larger Rf value then a less polar compound? Explain your answer by referring to TLC theory (stationary phase, moving phase, polarity). Thanks!
Problem 1 Thin layer Chromatography consist of three parts: The analyte, the stationary phase and mobile...
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Which of the following statements regarding thin layer chromatography are TRUE? Partition chromatography separates compounds based...
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1) Calculate the Rf value of a spot that travels 5.7 cm, with a solvent front...
1) Calculate the Rf value of a spot that travels 5.7 cm, with a solvent front that travels 13 cm. 2) A student spots an unknown sample on a TLC plate and develops it in pentane solvent. Only one spot, for which the Rf value is 0.05, is observed. Is the unknown material a pure compound? What can be done to verify the purity of the sample using thin-layer chromatography? 3) You try to synthesize n-hexyl bromide as a product...
A chemist runs a thin–layer chromatography (TLC) plate and, after development, forgets to mark the solvent...
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2. An unknown compound was analyzed using thin layer chromatography, its constituted of two components (A...
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Past paper question R, values for photosynthetic pigments may be determined using the technique of thin-layer chromatography (TLC). TLC plate Origin Solvent a. Outline what happens when spinach extract is spotted on a TLC plate and placed into a container of solvent. [2 marks] b. Explain what the Rf values represent in chromatography. [3 marks) c. State two photosynthetic pigments that could be identified using chromatography. [1 mark]
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What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is...
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1) Calculate the Rf value of a spot that travels 5.7 cm, with a solvent front that travels 13 cm. 2) A student spots an unknown sample on a TLC plate and develops it in pentane solvent. Only one spot, for which the Rf value is 0.05, is observed. Is the unknown material a pure compound? What can be done to verify the purity of the sample using thin-layer chromatography? 3) You try to synthesize n-hexyl bromide as a product...
2. An unknown compound was analyzed using thin layer chromatography, its constituted of two components (A and B) as shown in the TLC (Silica coated stationary phase; Chloroform and Ethyl acetate as mobile phase). The mixture was then loaded onto column with silica as the stationary phase and the same solvent system was used. (2 points) Column Chromatography TLC time A В Predict the order of elution of the compounds (A and B). which one would be eluted first/last and...
Past paper question R, values for photosynthetic pigments may be determined using the technique of thin-layer chromatography (TLC). TLC plate Origin Solvent a. Outline what happens when spinach extract is spotted on a TLC plate and placed into a container of solvent. [2 marks] b. Explain what the Rf values represent in chromatography. [3 marks) c. State two photosynthetic pigments that could be identified using chromatography. [1 mark]
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is based on analytes being partitioned between a stationary phase (not moving) and a mobile phase (moving). What is most commonly used as stationary phase in a column? Is this material polar or non-polar? In this experiment, you will use different solvents to run column chromatography separation of different pigments extracted from spinach leaves. These solvents are a mixture of acetone and hexanes. (a) What...
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