Thin Layer Chromatography (TLC) is a common technique that is routinely used in chemistry labs. It is simple, inexpensive, and very useful. Select the TRUE statement.
only second option correct.
lesser the polar more will be the Rf more the polar lesser the Rf
Thin Layer Chromatography (TLC) is a common technique that is routinely used in chemistry labs. It...
Which of the following statements regarding thin layer chromatography are TRUE? Partition chromatography separates compounds based on how they distribute themselves between the stationary and mobile phases. In thin layer chromatography, a polar organic compound will be attracted to the adsorbent and therefore travel further up the plate than a non-polar compound The Rf value is independent of the adsorbent material used. In thin layer chromatography, a polar organic compound will be repelled by the adsorbent and therefore travel not...
Hi can someone help me with my pre lab theory questions (1,2,3) on rate of elution or Rf? Here is some background below. Please help explain as many as possible (1-3)! I dont really get the relation ships in this lab, thank you! 1. What factors affect the rate of elution in organic compounds? 2. Explain what is the relationship between polarities of compounds (polar/non- polar compound) and rate of elution (Rf). 3. Explain what is the relationship between solvent...
Problem 1 Thin layer Chromatography consist of three parts: The analyte, the stationary phase and mobile phase. Match each of these terms to what is was in out experiment. Stationary Phase ____ a) The solvent Mobile Phase ____ b) Silica Analyte ____ c) One of the analgesiscs Problem 2 Complete the sentences Analytes on a TLC should have a(n) _________ Rf in a less polar solvent. Analytes on a TLC should have a(n) _________ Rf in a more polar solvent....
1. which of the following are not absorbent for TLC? a. magnesium silicate b. polyamides c. silica gel-F ( fluorescing indicator added) d. starch e. alumina f. none of the above 2. select the best answer from the statements below about thin layer chromatography A. in normal phase thin layer chromatography the stationary phase is polar silica gel b. in normal phase thin layer chromatography the mobile phase is an organic solvent or a mixture of organic solvents Which is...
In thin layer chromatography, would a more polar solvent increase or decrease the Rf values. Explain your answer by referring to TLC theory (stationary phase, moving phase, polarity...)
2. An unknown compound was analyzed using thin layer chromatography, its constituted of two components (A and B) as shown in the TLC (Silica coated stationary phase; Chloroform and Ethyl acetate as mobile phase). The mixture was then loaded onto column with silica as the stationary phase and the same solvent system was used. (2 points) Column Chromatography TLC time A В Predict the order of elution of the compounds (A and B). which one would be eluted first/last and...
In Thin Layer Chromatography Experiments, would a more polar compound give a smaller or larger Rf value then a less polar compound? Explain your answer by referring to TLC theory (stationary phase, moving phase, polarity). Thanks!
1. What is the purpose of Thin-Layer Chromatography (TLC)? 2. What are the phases involved in TLC? 3. What is coated on the TLC plate? 4. What is the purpose of lining the wall of the TLC chamber with paper? 5. Why is it important to use a small amount of the mobile phase? 6. Why should you remove the TLC plate before the mobile phase reaches the top? 7. What type of compounds are visible with UV light? 8....
In thin-layer chromatography, dhy do polar compounds require polar eluents to move them on the TLC plate?
Past paper question R, values for photosynthetic pigments may be determined using the technique of thin-layer chromatography (TLC). TLC plate Origin Solvent a. Outline what happens when spinach extract is spotted on a TLC plate and placed into a container of solvent. [2 marks] b. Explain what the Rf values represent in chromatography. [3 marks) c. State two photosynthetic pigments that could be identified using chromatography. [1 mark]