Question

Q11. when performing the Kirby-Bauer antibiotic test, the proper way to inoculate the bacteria on the agar plates is to (choose one)

a. use a sterile loop and streak for isolated colonies

b. use a sterile loop and inoculate using a zig-zag pattern on the plate

c. use a sterile swab and streak for isolated colonies.

d. use a sterile swab and cover the entire surface of the plate with the organism, creating a lawn of growth

Q3. The term auxotroph refers to a bacterial organism (choose one)

a. which can produce all necessary growth factors

b. which cannot produce all necessary growth factors

c. which can grow on GSA but not TSA

d. all of the above

e. b and c

f. a and c

Answer 1

11. d. use a sterile swab and cover the entire surface of the plate with the organism, creating a lawn of growth

In order to perform the Kirby Bauer antibiotic test, a bacterial suspension matching 0.5 Mcfarland is made and with a help of a steile swab dipped in the bacterial suspension, the entire MHA plate is covered with the organism in order to create a lawn.

3. b. which cannot produce all necessary growth factors

Auxotrophs are organisms that require an additional nutrient from outside source as they cannot manufacture it own their own.