Two cell lines (A and B) tested for viable cell numbers in the MTT assay at...
Two cell lines (A and B) tested for viable cell numbers in the MTT assay at confluency under identical conditions yield absorbance values of 0.90 and 0.63, respectively. Two reasons for this discrepancy
Homework Answers
MTT assays is a metabolic assay that rely on a mitochondrial reductase to convert the tetrazole to formazan. The assumption is that the conversion is dependent on the number of viable cells.
The reason for difference in absorbance value cane be - there can be instances where cells are still slowing metabolizing but not actively in division.
Many reductases and compounds in cell free culture can convert MTT to formazan,. Very healthy cells can also have a pretty low basal metabolic rate and give you low MTT production. So to say it shows cell death/proliferation can be a bit presumptuous.
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Two cell lines (A and B) tested for viable cell numbers in the
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(Round to two decimal places as needed.)
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