Homework Answers
Answer. The protein which is cationic in nature will interact with the column and will come out last. While the protein which is anionic in nature will have no interaction with coulmn and will come out fast. So, Protein A will come out first as there is interaction force in the column which is holding the protein and protein C which is cationic in nature will have cationic interaction with the column which will hinder its movement so it will come out in the last.
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If protein A has a pl of 3.1, protein B has a...
Biochem help 2 14. What would be the net charge on the dipeptide Ser-His at pH-...
Biochem help 2
14. What would be the net charge on the dipeptide Ser-His at pH- 6.04? (Choose the one best answer.) a) 1.5 b) +1; c) +0.5 d) 0 e) -0.5; 15. The pKa of a lysine side chain in a protein ending up on the outside of a globular protein has a different pKa than if the lysine is buried within the interior of a protein. What would be the expected pKa of a side chain of lysine...
(iln 2. Use the letters given to answer the questions below for full credit Protein name pl A Non...
(iln 2. Use the letters given to answer the questions below for full credit Protein name pl A Nonheme iron-containing fermitin (Pf) B Pynuvate: ferredoxin oxidoreductase, alpha Subunit (PorA) 44613 CGTP-binding proteia (Gtpl) D Co-chaperone (GiroES) E Adhesin-thiol peroxidase (TagD) FI Fumarase (Fund) 19,155 5.49 5.81 40,444 5.53 12,860 6.59 18,161 8.18 50,844 7.29 i) (5 points) Sketch the Isoelectric focusing gel after the separation of above proteins is performed. Clearly label everything needed for full credit. i) (5 points)...
need only part a and C. please and thank you 6. Suppose you have a mixture...
need only part a and C. please and thank you
6. Suppose you have a mixture of five proteins listed in the table below: Protein pl Mol. Welght, kDa Homer 4.6 69 Liza 10.7 13 Marge Maggie Bart 5 40 6.4 8.5 7 27 Indicate the order in which the proteins elute from a Bio-Gel p-10 gel-filtration column (starting with the on that elutes first). Is this the best resin to separate all the components of this mixture? Why? If...
a. Which spot corresponds to the protein with the largest molecular weight? b. When pH = 7, what order would the follow...
a. Which spot corresponds to the protein with the largest
molecular weight?
b. When pH = 7, what order would the following peptides elute
from a column filled with cation exchange resin? Size exclusion
column?
Protein 1 – 400 kDa;
10%A 5%E, 5%F, 10% G, 5% H, 10%I, 10%K, 10%L 10%R, 5%P, 5%S, 10%W,
5%Y
Protein 2: 40 kDa
5%A, 5%C, 5%D, 5%E, 5%F, 10%G, 5%I, 10%K, 5%L, 5%M, 5%N, 10%P, 5%R,
5%S, 5%T, 5%V, 5%W,
Protein 3 – 175...
please help with these 4 questions and explain why you chose what you did. thanks and...
please help with these 4 questions and explain why you
chose what you did. thanks and I'll give thumbs up.
11. A particular amino acid without an ionizable R-group would have a pl of is 3.1 and pK2 is 8.9 ; if its pK1 2.9 a. b. 5.8 6 C. d. 12 12. A certain amino acid with an ionizable side chain has a pK1 of 2.4, a pK2 of 8.9, and a pKR of 6.5. If the amino acid...
You are running an cation exchange column at pH 7.0. Your protein has a pI of...
You are running an cation exchange column at pH 7.0. Your protein has a pI of about 8.3 and the contaminating protein has a pI of about 7.5. Which of the following statements is most true? a. Both proteins will bind but your protein will bind more tightly b. both proteins will bind and the contaminating protein will bind more tightly c. neither protein will bind
What are the instruments that have been utilized for the review article discussions? ` 1. Introduction...
What are the instruments that have been utilized for the
review article discussions?
`
1. Introduction In recent years, nanoclays have been the object of particular interest for many scientists and researchers in chemistry, physics, engineering and biology due to their excellent properties as well as their sustain- ability [1-3]. For instance, they represent the starting point to the de velopment of smart materials for drug delivery (4-9), food packaging [10-12), environmental remediation and wastewater treatment [13], cultural heritage [14–17and...
Biochem help 2
14. What would be the net charge on the dipeptide Ser-His at pH- 6.04? (Choose the one best answer.) a) 1.5 b) +1; c) +0.5 d) 0 e) -0.5; 15. The pKa of a lysine side chain in a protein ending up on the outside of a globular protein has a different pKa than if the lysine is buried within the interior of a protein. What would be the expected pKa of a side chain of lysine...
(iln 2. Use the letters given to answer the questions below for full credit Protein name pl A Nonheme iron-containing fermitin (Pf) B Pynuvate: ferredoxin oxidoreductase, alpha Subunit (PorA) 44613 CGTP-binding proteia (Gtpl) D Co-chaperone (GiroES) E Adhesin-thiol peroxidase (TagD) FI Fumarase (Fund) 19,155 5.49 5.81 40,444 5.53 12,860 6.59 18,161 8.18 50,844 7.29 i) (5 points) Sketch the Isoelectric focusing gel after the separation of above proteins is performed. Clearly label everything needed for full credit. i) (5 points)...
need only part a and C. please and thank you
6. Suppose you have a mixture of five proteins listed in the table below: Protein pl Mol. Welght, kDa Homer 4.6 69 Liza 10.7 13 Marge Maggie Bart 5 40 6.4 8.5 7 27 Indicate the order in which the proteins elute from a Bio-Gel p-10 gel-filtration column (starting with the on that elutes first). Is this the best resin to separate all the components of this mixture? Why? If...
a. Which spot corresponds to the protein with the largest
molecular weight?
b. When pH = 7, what order would the following peptides elute
from a column filled with cation exchange resin? Size exclusion
column?
Protein 1 – 400 kDa;
10%A 5%E, 5%F, 10% G, 5% H, 10%I, 10%K, 10%L 10%R, 5%P, 5%S, 10%W,
5%Y
Protein 2: 40 kDa
5%A, 5%C, 5%D, 5%E, 5%F, 10%G, 5%I, 10%K, 5%L, 5%M, 5%N, 10%P, 5%R,
5%S, 5%T, 5%V, 5%W,
Protein 3 – 175...
please help with these 4 questions and explain why you
chose what you did. thanks and I'll give thumbs up.
11. A particular amino acid without an ionizable R-group would have a pl of is 3.1 and pK2 is 8.9 ; if its pK1 2.9 a. b. 5.8 6 C. d. 12 12. A certain amino acid with an ionizable side chain has a pK1 of 2.4, a pK2 of 8.9, and a pKR of 6.5. If the amino acid...
What are the instruments that have been utilized for the
review article discussions?
`
1. Introduction In recent years, nanoclays have been the object of particular interest for many scientists and researchers in chemistry, physics, engineering and biology due to their excellent properties as well as their sustain- ability [1-3]. For instance, they represent the starting point to the de velopment of smart materials for drug delivery (4-9), food packaging [10-12), environmental remediation and wastewater treatment [13], cultural heritage [14–17and...
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