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7. For each of the following initiation/elongation factors, how would eukaryotic translation be affected if it...
Which of the following eukaryotic mRNA 5' untranslated sequences is likely to interfere with translation initiation? A) 5'-AUAUAGAAAAUAACGCUUAUUAAUUCUUUGAAUG B) 5'-CGUUACAAAAUAACGCAUUUCCAAUUCUUUGAAUG C) 5-AUAUAGAAAAUAACGCAUUUCCAAUUCUUUGAAUG
* 1. Which of the following statements describes translation initiation in Eukaryotes: Initiation factors, a tRNAmet, and the ribosome's small subunit bind to the poly-A tail of mRNA. ✓ The ribosome's large subunit, tRNA, and P-site scan the mRNA for codons. ✓ The ribosome's large subunit binds to AUG codon on the mRNA. A ribosome binds to the C-terminus of an mRNA. Initiation factors, a tRNAmet, and the ribosome's small subunit bind to the 5'cap of mRNA. Initiation factors provide...
QUESTION 12 Which stage of transcription in eukaryotes requires transcription factors? a) initiation b) elongation c) termination d) initiation and elongation e) all of these
5. Circle all components of translation that dissociate upon the action of release factors (e.g. RF1/2 and RF3). 2 pts a. deacylated tRNA b. ribosomal subunits c. acylated tRNA d. polypeptide e. peptidyl transferase 6. GCN4 is a protein whose production is regulated at the level of translation by phosphorylation of the eIF2 α subunit. a. What interaction is affected when eIF2 is phosphorylated by an eIF2α kinase? 2 pts a. The interaction between eIF2 and a tRNA. b. The...
QUESTION 12 Which stage of transcription in eukaryotes requires transcription factors? O a) initiation Ob) elongation c) termination d) initiation and elongation e) all of these
1. Describe the differences between prokaryotic and eukaryotic translation initiation. How does the ribosome find the correct start codon and what proteins are involved in the process? please include the shine-dalgarno sequence in the answer. 2. Consider the following partial sequence of messenger RNA. The sequence below contains the code for a short, complete protein. 5 ́-UCCCCAGUCAUGGAGUCGUUAAUUAAAUGACCGGUGCGGAUCGUA - 3 ́ Using the codon chart (from your textbook or in the lecture slides), give the amino acid sequence of the protein...
Describe the steps (Initiation, Elongation and Termination) involved in translation of mRNA to generate a protein, including the all the important molecules involved and how they interact. Diagrams MUST be included in your answer. (Draw on some paper, then photograph and insert the drawing below.) You may add to your answer using bullet points if you find it easier, but make sure they are in the correct order!
6) Please describe the function of the Shine-Dalgarno sequence in prokaryotic translation initiation. (10 points) 7) During translation elongation in procaryotes, the interplay between EF-Tu and EF-TS regulate tRNA charging to the A site of the ribosome. Please describe in detail the sequence of events that take place during tRNAEFTU GTR ternary complex formation. Explain how the EF-Tu GDP that is discharged from the ribosome is converted to EF- Tu GTR that is the competent complex for translation elongation? What is...
Which of the following is not involved in the formation of a eukaryotic transcription initiation complex? a. TATA box b. transcription factors c. snRNA d. promoter
15. Translation (RNA protein) has three main stages: initiation, elongation, and termination. a. Initiation occurs when the small ___________________ subunit binds to the ____ end of mRNA and is then joined by the large _________________ subunit (which has three sites called the A, P, and E sites). Once the complex is formed, the _______________ begins to read the mRNA in a ____ to ____ direction. When it reaches the first start codon (_________) a tRNA carrying the amino acid ______________________...