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Buffered solutions (or simply buffers) are used in many processes in biochemistry/biotech labs. Many of the common buffers like phosphate buffered saline (PBS) or Tris/Boric Acid/EDTA BE) are commercially available as concentrated stock solutions at 10X the working concentrations. For the first buffer, PBS, the buffering system is a result of the equilibrium between the acid monobasic sodium phosphate (NaH2PO4) and its conjugate base, dibasic sodium phosphate (Na2HPO) if the equilibrium has a Keq of 3.98E-7 at the working temperature.
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avd Ka 3 .98 x 10 Answer . pka6y raud

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