Given the pH = 7.000, [buffer] = 0.0200 M, [NaCl] = 0.0600 M and Vol = 0.100 L - calculate the [acid], [base], [NaCl] and equivalent weights of acid, base and salt.
Given the pH = 7.000, [buffer] = 0.0200 M, [NaCl] = 0.0600 M and Vol =...
You are instructed to create 400. mL of a 0.40 M phosphate buffer with a pH of 6.4. You have phosphoric acid and the sodium salts NaH2PO4, Na2HPO4, and Na3PO4 available. (Enter all numerical answers to three significant figures.) H3PO4(s) + H2O(l) H3O+(aq) + H2PO4−(aq) Ka1 = 6.9 ✕ 10−3 H2PO4−(aq) + H2O(l) H3O+(aq) + HPO42−(aq) Ka2 = 6.2 ✕ 10−8 HPO42−(aq) + H2O(l) H3O+(aq) + PO43−(aq) Ka3 = 4.8 ✕ 10−13 Which of the available chemicals will you use...
You are instrucbed to create 900. mL of a 0.59 M phosphate buffer with a pH of 6.4. You have phosphoric acid and the sodium salts NaH2PO4, Na2HPO4, and Na3PO4 available. numerical answers to three significant H3PO4(s)H20)H3 aH2PO4(aq) H2PO4-(aq) + H2O(りーH3O + (aq) + HP042-(aq) Ka2-6.2×10-8 HPO()H20()H3)PO43 (aq) Ka 4.8x10-13 Ka 6.9x10-2 Which of the available chemicals will you use for the acid component of your buffer? O H3PO4 O Na2HPO Na3PO4 Which of the available chemicals will you use...
You are instructed to create 400. mL of a 0.39 M phosphate buffer with a pH of 6.2. You have phosphoric acid and the sodium salts NaH2PO4, Na2HPO4, and Na3PO4 available. (Enter all numerical answers to three significant figures.) H3PO4(s) + H20(I) =H30+ (aq) + H2PO4 (aq) Kai = 6.9x10-3 H2PO4 (aq) + H20(1) =H30+ (aq) + HPO42-(aq) Ka2 = 6.2 x 10-8 HPO42-(aq) + H20(1) = H30+(aq) + PO43-(aq) Ka3 = 4.8x10-13 Which of the available chemicals will you...
You are instructed to create 400. mL of a 0.39 M phosphate buffer with a pH of 6.2. You have phosphoric acid and the sodium salts NaH2PO4, Na2HPO4, and Na3PO4 available. (Enter all numerical answers to three significant figures.) H3PO4(s) + H2O(l) = H30+ (aq) + H2P04 (aq) Kai = 6.9 x 10-3 H2PO4 (aq) + H20(1) =H30+ (aq) + HPO42-(aq) Ka2 = 6.2 x 10-8 HPO42-(aq) + H20(I) = H30+(aq) + PO43-(aq) Ka3 = 4.8 x 10-13 Which of...
You are instructed to create 400. mL of a 0.39 M phosphate buffer with a pH of 6.2. You have phosphoric acid and the sodium salts NaH2PO4, Na2HPO4, and Na3PO4 available. (Enter all numerical answers to three significant figures.) H3PO4(s) + H20(1) =H30+ (aq) + H2PO4 (aq) Kai = 6.9x10-3 H2PO4 (aq) + H20() = H30+(aq) + HPO42- (aq) Ka2 = 6.2x 10-8 HPO42-(aq) + H20(I) = H30+(aq) + PO43-(aq) Ka3 = 4.8 x 10-13 Which of the available chemicals...
Sample Buffer Calculation: Given a pH of 5.4, prepare a buffer using one of the buffer systems below: System A: 3.5M acetic Acid (MM=60.1) : sodium acetate, anhydrous (MM=82.0) pKa = 4.74 System B: Sodium phosphate, monobasic (NaH2PO4 x H2O) (MM=138.0) : Sodium phosphate, dibasic, Na2HPO4 (MM=142.0) pKa = 6.86 a) Choose the buffer system most effective at pH of 5.4 b) Calculate the amounts of weak acid and conjugate base you need to make 25mL of 0.25M solutions (one...
Using the K values in the table below, calculate the pH of a buffer that contains the given concentrations of a weak acid and its conjugate base. 0.72 M Na HPO and 0.38 M Na PO pH Table 9.6 Common Buffers Buffer Weak Acid Conjugate Base к, Acetic acid/acetate CH-соон сн,соо" 1.8 x 10-5 Bicarbonate/carbonate HCO, co 5.6 x 10-11 Dihydrogen phosphate/ hydrogen phosphate H.PO HPO 6.2 x 10 Hydrogen phosphate/ phosphate HPO PO 2.2x 10-13
In lab, we have been using PBS. This is phosphate buffered saline. Phosphate Buffered Saline is usually 25mM to 100mM Phosphate Buffer with 137mM NaCl added and often at pH 7.4. You need to make 1L of 50mM PBS. The buffer is 50mM and the salt remains at 137mM. You have the following reagents on the shelf: - NaCl (MW = 58.44 g/mole); - Sodium phosphate dibasic heptahydrate (Na2HPO4 · 7H2O; MW = 268 g/mole) - Sodium phosphate monobasic...
Pre-Lab Assignment Hydrogen Phosphate Buffer System This pre-lab assignment is mandatory. You will receive a zero for the experiment if the pre-lab assignment is not completed before the beginning of the lab session. 1. Calculate the masses of the conjugate acid and base needed to prepare each of the six buffer solutions listed in the table on the left. (p of H,PO,= 6.70) Assume the availability of the phosphate salts listed in the table on the right. Fill out the...
Physiological buffers can be prepared by combining the base HPO4 with its conjugate acid H2P0,- (pk, -7.21) as per the equilibrium shown below. H3PO,- (aq) 2 HPO 2- (aq) + H+ (aq) Calculate the molar ratio of (HPO 2-Y(H2PO4) needed to prepare a buffer with pH = 7.40 Calculate the grams of Na2HPO4 (141.96 g/mol) required to prepare a buffer of pH = 7.40 if 12.584 g of NaH,PO(119.98 g/mol) are available.