3) EMB is an undefined selective/differential medium. It contains aniline dyes, which inhibit the development of Gram-positive bacteria and selecting for Gram-negative bacteria. It also contains lactose which creates the media differential that is based on ability of organisms to ferment lactose. It consists of a dark blue/green to dark purple agar to promote the development of members of the family Enterobacteriaceae.
4) Eosin Y and methylene blue are pH indicator dyes which combine to form a dark purple precipitate at low pH. They inhibit the development of most Gram positive organisms. They forms a dark purple precipitate and absorbed by the cytoplasmic material of the bacterial cells at low pH.
5) lactose acts as fermentable carbohydrate sources. It promotes the development of gram negative fecal coliforms. Lactose fermentation produce a low pH condition which helps the dyes to form the complex and to differentiate the bacteria from the weak fermentors which produce mucoid or pink colonies.
agar a defined or an undefined medinent Provide the reasoning behind your choice and explain wry...
4-6 Eosin Methylene Blue Agar (Levine) OBSERVATIONS AND INTERPRETATIONS Refer to Table 4-6, page 268, when recording your results and interpretations in the table below. Use abbreviations or symbols as needed. Growth (N/P/G) EMBINA Organism EMB Growth Color (Pi/D/C) Interpretation and Presumptive ID QUESTIONS I in your own words, what is the application (purpose) of EMB agar? 2 Which ingredient(s) in EMB agar supply(ies) a. Carbon b. Nitrogen? ur choice and explana 3 Is EMB agar a defined or an...
Think about the results you recorded. a. Growth on the PEA and NA plates was recorded as "good growth," "p oor growth," or “no are qualitative and, at least for the first two, subjective terms. What did you use to establish what These constituted "good growth?" bhy wouldn't it be advisable to compare growth of the organisms on each plate to each other? There are at least two answers to this question!
Is HE agar a defined or an undefined medium? Provide the reasoning behind your choice and explain why this formulation is desirable.
microbiology help TOT Zoo Add Page Insert Table Chart Text Shape Media Comment These questions will serve in lieu of a lab report for Exercise 15, 16, and 17 You will find the answer to these questions in the background, procedure, results and interpretation sections of manual Exercise 15, 16, and 17, videos, Actions of Selective and Differential Media Chart, and the Principle/Theory article in homework section.) General Questions 1. What is the purpose (function) of selective media? (How does...