Question

pls answer 1 3 and 5

You prepared a 0.8% gel for this experiment, what effect would pouring a higher percentage (1-2%) gel have on the migration of the DNA through the gel? 1、 2. Describe the blood disease sickle cell anemia and how it affects its sufferers ntechens and daayd gaaih If the ß chain of human hemoglobin is 146 amino acids in length, calculate the minimum number of nucleotide base pairs needed to code for B-globin. How many mRNA codons does this correspond to? 3. Define RFLPs and give their significance. 4. sms ore manisestathcn Potmepis cren From the electrophoresis experiment, could these DNA samples have been distinguished from one another if another restriction enzyme, such as EcoRI had been 5. used? Exercise 10 TAgarose Gel Electrophoresis: Post-Lab 151

Answer 1

1 As the percentage of agarose gel used is increased,the migration rate decreases ,resulting in the resolution of smaller DNA fragments.Conversely ,as the percentage of agarose gel used is decreased ,migration rate  also increases resulting in resolution of larger DNA molecules.

3   20 amino acids are encoded by combinations of 4 nucleotides

number of nucleotide base pairs required = 29

number of mRNA codons this corresponds to = 438

(146*3)=438 because 3 nucleotides make up 1 codon, which codes for the amino acid.

5 Yes.Restriction enzymes are used to separate DNA samples in electrophoresis.

After the restriction enzyme cuts the DNA, the fragments are of different lengths and can be separated via

Electrophoresis.