Question

Which combinations of enzymes in the table leave compatible ends (ends that can be ligated together)? The symbol indicates the cleavage site. Check all that apply. Re tion Enzyme Recognition Site AT TAAT sp 1208l AT CGAT CGGCCG GG CGCC CA TATG ag Ner l Nori TAAT TAA PstI PvuI eo CTGCA G Check Al Thet Apply Cla l, Narl, and Taql Ase I and Nde l Eag I and Notl 。Pac l and Pvul Bsp 12861 and Pst I

Answer 1

restriction site of EagI is 5`CGGCCG 3` and it cuts after first C leaving 5`GGCCG 3` and for Not I 5` GCGGCCGC3` it cuts after C so leaving 5` GGCCGC3` so it complementary strand will be 3`CCGGCG 5` it can pair with 5`GGCCG 3` which is the restriction site of Eag I.

restriction site os Bsp12861 is GTGCAC it cuts before C so it leaves 5`GTGCA 3`and when PstI cuts it leaves 5`CTGCA 3`

so the complementary strand of 5`GTGCA 3` is 3`CACGT 5` can pair with 5`CTGCA 3` so

(EagI and Not I) and (Bsp12861 and PstI) are the answers.