Question

Explain the basic process of PCR. Include: what starting materials are required and what each of them does, tell me each of the steps in the PCR cycle and what the purpose is for each.

Answer 1

PCR stand for Polymerase Chain Reaction.This process was designed by K.Mullis....It is used for the amplification of gene of interest (~1 billion time).High temperature about 95°C is required for seperatios of the two strand of the target DNA and this step is called Denaturation..DNA primers bind to the end of the DNA strands at 40°C to 60°C and this step is called Annealing.Taq Polymerase from Thermus acquaticus(a thermophilic bacterium) and deoxyribonucleotides (as a raw material) used to synthesize complementary copy of each strand at 70°C and this step is called Extension.And finally we got an amplified material.