Question

Using Agarose Gel Electrophoresis to identify Hemoglobin Phenotypes 13. Draw the banding patterns you obtained on the space below. Sample 1. A Banding pattern Well 1.0 lbo 2. AS 3. S 4. Unknown 6. O 7. 5 7.0 8. Unknown 8. O 14. Why does sickle cell hemoglobin behave differently from normal hemoglobin during agarose gel electrophoresis? 15. + A child is born with sickle cell disease. What can you conclude about the potential genotypes of the parents? 16. + A mother finds out that her son is red-green color-blind. She is not color-blind. What can you conclude about her genotype?

Answer 1

Question 14.

Normal hemoglobin are more negatively charged.

Mutation it changes the overall charge of the sickle cell hemoglobin and therefore the sickle cell hemoglobin band forms in a different place in the gel than normal hemoglobin

Question 15.

The child both parents are heterozygous for sickle cell trait.

Both could be an carrier.

Question 16.

Mother must be an heterozygous for color blind trait.

Mother has normal vision but she must be a carrier for the red-green color-blindness.