Question

7. Once isolated in the laboratory, Staphylococcus can be easily distinguished from Streptococcus on the basis of: a. The catalase reaction b. The oxidase reaction C. The lactose fermentation reaction d. The glucose fermentation reaction 8. The use of chemical agents on skin or living tissue to inhibit or eliminate microbes is known as: a. Sterilization b. Antisepsis C. Disinfection d. Pasteurization 9. What is the total magnification for oil immersion? a. 10 b. 400 O 1000 d. 50 10. Why is blood agar used as media? a. Selective media b. For nutrients not found on regular general purpose media C. To visualize hemolysis and differentiate d. All of the above 11. What are things to do while using a Bunsen burmer? a. Have hair down and near Bunsen burner b. Never lean over burner with flame on c. Light match before tuming gas flow on d. Kill the pregnant woman! Part II. Match the following accordingly: A. Anaerobic Identification of S. aureus. B. Selective _Screening of pathogenic enteric gram-negative rods. C. Differential Differentiation of Streptococcus pyogenes from other streptococci. D. Enriched Differentiation of Group D enterococci from Group D non-enterococci. E. Aerobic Differentiation of Streptococcus agalactiae from other streptococci. F. Rapid agglutination test Mycobacterium tuberculosis Encapsulated microorganisms G. Kinyoun stain Enterobacteriaceae H. India Ink Microorganism that grows in the presence of O2 1. Bile-esculin and 6.5% salt medium Microorganism that grows in the absence of O. J. Carbohydrate fermentation Medium used to distinguish between bacterial species or groups. K. Inhibition of growth by bacitracin Medium containing substances that stimulate a single organism or L. Fermentation of lactose groups of organisms to grow more readily. M. Production of CAMP factor _Medium that allows the growth of certain bacteria while inhibiting the N. Growth in 6.5% salt broth growth of others. 0. Agglutination with anti-protein A antibodies Page 2 of 3

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Answer 1

7. Catalase reaction.

Staphylococus show positive catalase test and streptococcus shows negative test.

8. Antisepsis.

9. C. 1000.

Oil eliminates the two refractive surfaces and thus magnification of 1000X or greater can be achieved.

10. C. To visualise haemolysis and differentiate.

Blood agar differentiates pathogenic bacteria based on the haemolysins which lyse red blood cells.

11. Never lean over burner with flame on