How is the reductase is regulated by the activity and specificity sites on R1? Includes the overall sequence for how the reductase changes its substrate specificity from pyrimidines to GDP then to ADP.
HMG-CoA reductase, a critical enzyme in cholesterol synthesis is the target or statin inhibitors. You are characterizing a possible new inhibitor and want to determine its mode of binding to HMG-CoA reductase. Equilibrium dialysis measurements at 25°C, 30°C, and 37°C yielded dissociation constants (Ks) of 2.5 x 10* M, 1.5 x 10*, and 1.0 x 10* M, respectively, for the HMG- CoA reductase-inhibitor complex a. Is the binding becoming better or worse with increasing temperature? Explain. b. Using Excel or...
HMG-CoA reductase, a critical enzyme in cholesterol synthesis is the target or statin inhibitors. You are characterizing a possible new inhibitor and want to determine its mode of binding to HMG-CoA reductase. Equilibrium dialysis measurements at 25 C, 30°C, and 37 C yielded dissociation constants (K.) of 2.5 x 10" М, 1.5 x 10", and 1.0 x 103 M, respectively, for the HMG-CoA reductase-inhibitor complex. a. Is the binding becoming better or worse with increasing temperature? Explain. b. Using Excel...
14. Describe how the synthesis of nucleotides is regulated. 14. Describe how the synthesis of nucleotides is regulated.
Rachel is studying cholesterol synthesis in mice. Some mice had a mutation in their sterol regulatory element binding protein (SREBP) that is normally active when cholesterol levels are low in the cell. This mutation can no longer bind to the gene promoter for cholesterol synthesis pathway enzyme HMG-CoA reductase. What would happen to HMG-CoA reductase levels? Again, identify this type of effect and briefly define it.
QUESTION 4 The rate limiting enzyme for cholesterol synthesis is A Acyl-CoA-cholesterol acyl transferase (ACAT) B. HMG-CoA Lyase C. HMG-CoA Reductase D. HMG-CoA Synthase
Statin drugs, like Mevacor (lovastatin) and Lipitor (atorvastatin), act as competitive inhibitors of HMG-CoA reductase by mimicking the structure of mevalonate. However, it is not this inhibition that directly lowers cholesterol levels in the blood. What enzymatic reaction does HMG CoA-reductase catalyze? What is important about this step of cholesterol synthesis? How can statins mechanistically cause a decrease in LDL cholesterol in the blood if it is not a direct effect of decreased cellular cholesterol synthesis?
SERBP, SCAP, Insig and Sec are proteins associated with regulating the synthesis of HMG-CoA reductase and other enzymes of cholesterol synthesis and uptake. What compounds bind to SCAP and Insig to down-regulate HMG- CoA reductase and cholesterol synthesis? A. ammonia and urea, B. glutamate and glutamine, OC. sterols and oxysterols, OD IMP and OMP, O E. all of the above, OF none of the above, QUESTION 15 The synthesis of which of the following compounds requires the most ATP and...
1-Explain how the synthesis of fatty acids is regulated (35 points). 2-Explain how beta-oxidation and fatty acids synthesis are reciprocally regulated (30 points). . 2- Explain how insulin and glucagon regulate lipolysis (35 points).
Fatty acid synthesis is regulated in 2 ways. What are they, how do they function, and which one contains the "committed step"?