How did the temperature affect the pH of your buffer? What are the implications for a...
How did the temperature affect the pH of your buffer? What are the implications for a buffer you plan to use in the cold room (at ~4°C)? Buffer: 1.5M Tris-HCl, pH=8 (15mL 0.75M HCl+ 18.171g Tris + 35mL water) --> 100mL sol'n
Homework Answers
ANSWER
The pH of a buffer change with temperature, hence it is advisable to set the pH at working temperature. As the temperature increases the dissociation increases i.e. the H+ will increase in the buffer. This results in decrease in pH. Hence pH at 37oC will be lower than at 4oC.
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How did the temperature affect the pH of your buffer? What are
the implications for a...
Calculate how you will prepare 0.1M buffer solutions ranging from pH 1-12 in increments of 1...
Calculate how you will prepare 0.1M buffer solutions ranging from pH 1-12 in increments of 1 pH unit. NaOH and HCl droppers will be available to make adjustments to the final pH. a. pH 9 Tris Base (pKa 8.08, 121.14g/mol) and Tris-HCl (151.59g/mol) are available. b. pH 10, 11, 12. CAPS (3-(Cyclohexylamino)-1-propanesulfonic acid, 221.32g/mol, pKa 10.4) is available. c. pH 7, 8. Dibasic potassium phosphate (pKa 6.82, 174.18g/mol) and monobasic potassium phosphate (136.09g/mol) are available
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1) Write out your calculations for preparing 2% SDS lysis buffer (use M1V1=M2V2) 2) Based on...
1) Write out your calculations for preparing 2% SDS lysis buffer
(use M1V1=M2V2)
2) Based on these calculations, how many micrograms of protein
are in 10 microliters of each sample?
In an Eppendorf tube, prepare 1 mL of 2% SDS lysis buffer (M1V1 = M2V2) 2% SDS lysis buffer 1 mL-1 2% SDS (stock = 20% 50 mM Tris-HCl, pH 8 (stock = 1 M) MV, = M,V2 10 mM EDTA (stock = 0.5 M) Bring up to Vf of...
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8) A buffer used to denature proteins incudes the following components: 25 mM Tris-HCI, pH 8.2...
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You need to make a buffer solution with pH of 8.0. You have the following reagents...
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1. The process of calculating the % alpha-helix and beta sheets in a protein is called...
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1. Show your working out for the following calculation a) TENS buffer:  
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Given: pH: 7.60 Concentration (M): 0.050 mL: 100mL Determine the Mass of Each Component Recall that...
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1) Write out your calculations for preparing 2% SDS lysis buffer
(use M1V1=M2V2)
2) Based on these calculations, how many micrograms of protein
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In an Eppendorf tube, prepare 1 mL of 2% SDS lysis buffer (M1V1 = M2V2) 2% SDS lysis buffer 1 mL-1 2% SDS (stock = 20% 50 mM Tris-HCl, pH 8 (stock = 1 M) MV, = M,V2 10 mM EDTA (stock = 0.5 M) Bring up to Vf of...
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