Question

Q7. Chorismate mutase from E. coli catalyzes the Claisen rearrangement of both chorismate and the O-methyl derivative 1 with the kinetic parameters below. (4 points) CO2 Km(mM) car (s Chorismate R-H 0.14 Derivative 1 R CH3 1.9 29 0.56 A. Which substrate binds more tightly? B. Which bound substrate rearranges more rapidly? C. Overall, which substrate is rcarranged more quickly by the enzyme?

Answer 1

A) Chormistate bonds more tightly ( since low Km value)

B) Chormistate substrate rearranges more quickly ( since high Kcat value)

C) Overall chromistate is reaaranged more quickly by enzyme