Consider the cross: Hfr: strS arg+ met+ gal+lac+lys- x F-: strS arg- met- gal-lac- lys+ where the genetic map is shown below (the < indicates the Origin of transfer in the Hfr strain).
a.
Minimal media + galactose + streptomycin + arginine + lysine + methionine + lactose
b.
Minimal media + galactose + arginine + methionine + lactose
c.
Minimal media + galactose + arginine + lysine
d.
Minimal media + galactose+ arginine + lactose
e.
Minimal media + streptomycine+ arginine + lysine + lactose
f.
Minimal media + galactose+ arginine + methionine
g.
More than one of these above
h.
None of these above
Which media would allow the growth of the Hfr strain?
The correct answer is c.
I see the correct answer, but I do not know why methionine is not included in the plate. I thought we leave amino acids on the plate if we are not trying to kill the parental
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Please explain why the answer is h.
1. Consider the cross: Hfr: str erys arg+ met+ gal-lac-lys- x F:stre eryk arg-met-gal+lac+ lys+ where the genetic map is shown below (the < indicates the Origin of transfer in the Hfr strain). arg gal ery str met lac Circle the media below which would select for the most exconjugants. a) Minimal media + arginine b) Minimal media + streptomycin c) Minimal media + galactose + methionine + lysine d) Minimal media +...
BIS 101 Homework 04 Dr. Crowder nexperiment is performed in Escherichia coli using the parents Hfr arg str and Farg lys met his stor. The mating was interrupted at several d on several different media with the following results. The table below shows the number of colonies obtained on different media as es obtained on different media as indicated. a Minimal medium plus streptomycin plus Histidine Arginine Arginine Lysine Histidine Histidine Methionine Methionine Lysine ine Arginine Lysine Methionine Time (min.)...
1) Genetics a) Which of the following genotypes have one haplotype in common with the genotype aBcdE/ABcDe? abdeE/ABcDE ABcDE/ABCDe abCDE/aBcdE ABcde/abcde b) Consider an experiment in which an Hfr strain of E. coli bacteria with the StrS, his+, met+ alleles in its genome is mixed with an F- strain of E. coli with the StrR, his-, and met- alleles in its genome. After the cells have been cultured together for 8 hours, the mixture of cells is plated on minimal...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
4. (3) Suppose that you mate a lac+ Hfr strain with the genotype str S i+ o+ z+ y+ (that you grew in the absence of lactose) with a lac - F - strain with the genotype str R i- o+ z - y. You allow sufficient time for the transfer of the genes indicated, with the exception of str S , and plate the bacteria on minimal medium supplemented with arabinose and streptomycin but no lactose. You observe transient...
can someone explain how to draw a chromosome map based on this
data. the answer is
str, lac, arg
I am having difficulty in understanding this, if possible
could someone explain how to solve?
Time before conjugation was interrupted (min) % of cells that grow on MM + lactose + arginine + ampicillin % of cells that grow on MM + arginine + streptomycin + ampicillin 0 15 % of cells that grow on MM + ampicillin 0 0 10...
I don't quite understand how to answer this question can
someone please explain it?
You perform a conjugation experiment using Hfr lys+his+gly + amp with F-lys-his-gly -amp and you observe the following Minimal Medium with lysine and glycine ampicillin 40 CFU Minimal Medium with histidine and glycine ampicillin 410 CEU Minimal Medium with lysine and histidine ampicillin 150 CFU 14) not let buti la n What is the order of gene transfer? lysine, glycine, histidine What is the purpose of...
table for reference for question 4 table
h ell-free synoms produce Golshopecule consisting of a string of urucille RNA free systems produced the polypeptide poly string of phenylalanine amino acids phenylalanine ed entymes to produkce RNA polymers with they leotide. These polymers allowed them to inter to signal "start" and is therefore the art codon. In this case the as a dual function because also encodes the mind methionine (Met). You can see that 61 codons are more than enough...