1) Describe the differences between dry packing of the column like in the procedure and the...
1) Describe the differences between dry packing of the column like in the procedure and the wet method described in the video. Give two advantages and two disadvantages of each method for packing.
2) In the lab manual and the video tutorial, silica gel is used as the preferred stationary phase. Can you name two more options for a stationary phase? Discuss two advantages and two disadvantages compared to silica gel.
Homework Answers
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1) Describe the differences between dry packing of the column
like in the procedure and the...
Describe the differences between paper chromatography and TLC. Give two advantages and two disadvantages of each...
Describe the differences between paper chromatography and TLC. Give two advantages and two disadvantages of each method for analysis. In order to prepare for a column chromatography separation of 3 ink dye components, you have run a TLC experiment with three possible solvent mixtures. These are the results: Acetone:Hexane in 1:1 ratio gave the following distances: solvent=4.2cm, pigment #1=3.8cm, pigment #2=1.7cm, and pigment #3=1.3cm Acetone:Hexane in 2:1 ratio gave the following distances: solvent=4.3cm, pigment #1=3.8cm, pigment #2=2.1cm, and pigment...
1.) Describe the goals of a Gel Filtration Chromotography Experiment??? 2.) Explain each key theoretical principle...
1.) Describe the goals of a Gel Filtration Chromotography
Experiment???
2.) Explain each key theoretical principle of a Gel Filtration
Chromotography, and how they help acheive the goal???.
4.) Explain the key equations used in the Gel Filtration
Chromotography experiment and the terms involved in the
equation????
HI im trying to prepare for a lab/report and i have some
questions i could use help with please :) over all having trouble
seeing how everything ties together etc :) thank you...
-Fohandl NO Cont. Lat Exercise 5-Smear Preparation Procedure to prepare a smear(from liquid and solid media)...
-Fohandl NO Cont. Lat Exercise 5-Smear Preparation Procedure to prepare a smear(from liquid and solid media) - be able to correctly order all steps. Know which tools to use for making the smear. What is a target circle? Why is important to draw it? Why is it important to completely air dry the smear before heat fixing? What are two most important things that occur in heat fixing? Advantage/disadvantage of heat fixing Exercise 6-Simple Staining Basic -Cationic(+) vs. Acidic Dyes...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column /...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column / planar) The first step consists of the extraction of drug metabolites from biological fluids when we place the sample in a test tube containing a mixture of solvents and buffering salts that cause the extraction of basic and neutral drugs. The solvent extract of the sample is concentrated by heat and evaporation, then is deposited onto a small disc of chromatographic media (small circle...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy (EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
The following questions are from an experiment titled Friedel-Crafts Alkylation for Organic Chemistry II Lab. There...
The following questions are from an experiment titled
Friedel-Crafts Alkylation for Organic Chemistry II Lab. There are
pictures attached of the lab manual.
2) Is this method a good route for the preparation of
p-sec-butyltoulene? Give reasons for your answer.
3) Why do you use a large excess of toulene. What are its
functions?
4) What is the purpose for adding the hydrochloric acid to the
water in the separatory funnel in step 5? Hint: If no acid is
added,...
MODULE 15 EFFECTS OF DISINFECTANTS AND ANTISEPTICS OBJECTIVES Be familiar or be able to describe 1)...
MODULE 15 EFFECTS OF DISINFECTANTS AND ANTISEPTICS OBJECTIVES Be familiar or be able to describe 1) Antimicrobial substances: Chemical and biological 2) Bacteriocidal versus Bacteriostatic chemicals 3) Sterilization versus disinfection by chemical antimicrobials 4) Difference between disinfectants, antisepties, and antibiotics in terms of a. Antimicrobial activity b. Where they would be used c. Role of organic matter in their effectiveness d. Differential effects on Gram positive and Gram negative bacteria. 5) Phenol coefficient test 6) Disk Diffusion method of assessing...
please answer all prelab questions, 1-4. This is the prelab manual, just in case you need...
please answer all prelab questions, 1-4.
This is the prelab manual, just in case you need background
information to answer the questions. The prelab questions are in
the 3rd photo.
this where we put in the answers, just to give you an
idea.
Lab Manual Lab 9: Simple Harmonic Oscillation Before the lab, read the theory in Sections 1-3 and answer questions on Pre-lab Submit your Pre-lab at the beginning of the lab. During the lab, read Section 4 and...
For part 1 of this lab) I collected a soil sample from my campus Part 2)...
For part 1 of this lab) I collected a soil sample from my campus
Part 2) Tested bacteria initial viability Part 3) DNA extraction
Part 4) DNA quantification by Nanodrop Part 5) Sample sequencing
Part 6) PCR amplification Part 7) Gel electrophoresis Part 8) DNA
sequence data analysis (sent sample to another lab)
Directions for Part 3 DNA extraction are in the attached
image
QUESTIONS REGARDING PART 3 (DNA extraction)
1) What type of conclusions can be made from initially...
1. Focusing on only the inpatient care cost (i.e., ignoring operating room costs), what is the...
1. Focusing on only the inpatient care cost (i.e., ignoring
operating room costs), what is the cost of a TAH (non-oncology)
under each of the cost accounting systems? A tuboplasty? A TAH
(oncology)? What accounts for the differences?
Croswell University Hospital This report doesn't describe where our costs are generated. We're applying one standard to all patients, regardless of their level of care. What incentive is there to identify and account for the costs of each type of procedure? Unless...
1.) Describe the goals of a Gel Filtration Chromotography
Experiment???
2.) Explain each key theoretical principle of a Gel Filtration
Chromotography, and how they help acheive the goal???.
4.) Explain the key equations used in the Gel Filtration
Chromotography experiment and the terms involved in the
equation????
HI im trying to prepare for a lab/report and i have some
questions i could use help with please :) over all having trouble
seeing how everything ties together etc :) thank you...
-Fohandl NO Cont. Lat Exercise 5-Smear Preparation Procedure to prepare a smear(from liquid and solid media) - be able to correctly order all steps. Know which tools to use for making the smear. What is a target circle? Why is important to draw it? Why is it important to completely air dry the smear before heat fixing? What are two most important things that occur in heat fixing? Advantage/disadvantage of heat fixing Exercise 6-Simple Staining Basic -Cationic(+) vs. Acidic Dyes...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column / planar) The first step consists of the extraction of drug metabolites from biological fluids when we place the sample in a test tube containing a mixture of solvents and buffering salts that cause the extraction of basic and neutral drugs. The solvent extract of the sample is concentrated by heat and evaporation, then is deposited onto a small disc of chromatographic media (small circle...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy (EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
The following questions are from an experiment titled
Friedel-Crafts Alkylation for Organic Chemistry II Lab. There are
pictures attached of the lab manual.
2) Is this method a good route for the preparation of
p-sec-butyltoulene? Give reasons for your answer.
3) Why do you use a large excess of toulene. What are its
functions?
4) What is the purpose for adding the hydrochloric acid to the
water in the separatory funnel in step 5? Hint: If no acid is
added,...
MODULE 15 EFFECTS OF DISINFECTANTS AND ANTISEPTICS OBJECTIVES Be familiar or be able to describe 1) Antimicrobial substances: Chemical and biological 2) Bacteriocidal versus Bacteriostatic chemicals 3) Sterilization versus disinfection by chemical antimicrobials 4) Difference between disinfectants, antisepties, and antibiotics in terms of a. Antimicrobial activity b. Where they would be used c. Role of organic matter in their effectiveness d. Differential effects on Gram positive and Gram negative bacteria. 5) Phenol coefficient test 6) Disk Diffusion method of assessing...
please answer all prelab questions, 1-4.
This is the prelab manual, just in case you need background
information to answer the questions. The prelab questions are in
the 3rd photo.
this where we put in the answers, just to give you an
idea.
Lab Manual Lab 9: Simple Harmonic Oscillation Before the lab, read the theory in Sections 1-3 and answer questions on Pre-lab Submit your Pre-lab at the beginning of the lab. During the lab, read Section 4 and...
For part 1 of this lab) I collected a soil sample from my campus
Part 2) Tested bacteria initial viability Part 3) DNA extraction
Part 4) DNA quantification by Nanodrop Part 5) Sample sequencing
Part 6) PCR amplification Part 7) Gel electrophoresis Part 8) DNA
sequence data analysis (sent sample to another lab)
Directions for Part 3 DNA extraction are in the attached
image
QUESTIONS REGARDING PART 3 (DNA extraction)
1) What type of conclusions can be made from initially...
1. Focusing on only the inpatient care cost (i.e., ignoring
operating room costs), what is the cost of a TAH (non-oncology)
under each of the cost accounting systems? A tuboplasty? A TAH
(oncology)? What accounts for the differences?
Croswell University Hospital This report doesn't describe where our costs are generated. We're applying one standard to all patients, regardless of their level of care. What incentive is there to identify and account for the costs of each type of procedure? Unless...
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