Question

Explain two ways in which microbiologists have overcome the difficulty of viewing microbes in wet preparations

Answer 1

First we have to find two ways in which microbiologists have overcome the difficulty of viewing microbes in wet preparations are a bacteria which has the intrinsic ability of movement in the surrounding medium in which it remains suspended is a motile bacteria.

A bacteria which does not have the intrinsic ability of movement in the surrounding medium in which it remains suspended is a non-motile bacteria.

In wet mount though the shape and size of bacteria can be observed motility may be hampered as the suspension is pressed between the slide and the cover slip.

Motility test is performed for clear observation of the motility of bacteria, besides their shape and size.

The hanging drop is observed under a microscope using oil immersion objective its cells can be seen to have erratic movement in the surrounding medium.

If it is non motile its cells remain static in the medium without any movement or may show brownian movement resulting from the bombardment by the water molecules in the medium.