Question

5) Translation was originally sorted out using a variety of inhibitors of protein synthesis Inhibitors tend to interrupt the process at a particular step and a population of translating ribosomes will all stop in the same state. An allied technique is to identify temperature sensitive mutations in various accessory factors. When the population of ribosomes is shifted to the restrictive temperature, ribosomes will stall when they need the function of the mutant protein to continue By analyzing the structure of the interrupted complexes formed using both of these techniques, one can form a picture of the process. am not going to ask you to do that. Rather, am going to ask you, knowing as you do the steps in the translation process, to diagram the stalled complex that would result from the addition of various inhibitors or in the presence of some temperature sensitive mutations. As an aid to both of us I am asking you to use the representations below in your diagrams Do not include any initiation or elongation factors even if the question refers to them To illustrate what I want, I have done the first one as a sample (4 pts each) Met a a a a a a Small Large nitiator Charged tRNA with Release Ribosomal Ribosomal tRNA tRNA growing Factor Subunit subunit peptide mRNA A) Streptomycin prevents release of the uncharged B) Fusidic acid prevents the release of EF-G tRNA from the P site following peptidyl transfer from the ribosome following translocation. Met C) Kirromycin blocks the release of EF-Tu from the D) A temperature sensitive mutation in EF-Ts ribosome which blocks binding of EF-Ts to EF-Tu-GDP E) A temperature sensitive mutation in IF-3 that F) A temperature sensitive mutation in RF-3 prevents its dissociation from the initiation that prevents it from hydrolyzing GTP complex

Answer 1

Ans. B

Ans. C

Ans. D

Ans. E

Ans. F