Why is it significantly important to make sure your spotted analytes are above the level of...
Homework Answers
In Thin Layer Chromatography (TLC), the stationary phase is a glass plate on which an adsorbent material (for example silica gel, alumina) is applied, the analyte is spotted on the plate on the origin line and the plate is dipped in the mobile phase (the solvent front also called eluent) slightly below the origin line.
The spotted analytes have to be placed above the level of the eluents (mobile phase i.e. solvent front) when developing a TLC plate so that the spotted analyte doesn't get washed away in the eluent before even rising up the TLC plate.
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Why
is it significantly important to make sure your spotted analytes
are above the level of...
I need help with my pre and post lab questions. The three compounds below were spotted...
I need help with my pre and post lab questions.
The three compounds below were spotted on a TLC plate and developed to give the TLC plate shown. Which compound corresponds to which spot? Calculate the Rf value for spot B in the TLC plate shown above. When using polarity to determine the relative rates at which organic compounds travel up the TLC plate, we are considering compounds of similar molecular weight. It should also be noted that Rf values...
Why is it important to keep adequate records and make sure that your billing is correct?...
Why is it important to keep adequate records and make sure that your billing is correct? Why is this so important to the doctor you work for? Why is this so critical to the doctor’s success in the clinic? Your responsibility as a Medical Office Assistant is made up of several things. Taking care of the doctor, the patients, the office, the mail, faxes and so on. Billing is the one responsibility that is first and foremost. What makes this...
Classify and breifky explain the different patterns of genetic inheritance in humans Questions for the Thin...
Classify and breifky explain the different patterns of genetic
inheritance in humans
Questions for the Thin Layer Chromatography Laboratory 1. Why can't you use a pen to mark the origin of a TLC plate? Be explicit in your answer. 2. You were impatient to see how long it would take for your sample to resolve on the TLC plate, so you constantly removed the top from the chamber. Please explain why this is an incorrect practice. 3. What happens when...
1. When separating mixtures using TLC, why is it important to choose a solvent (mobile phase)...
1. When separating mixtures using TLC, why is it important to choose a solvent (mobile phase) that has a different polarity than the TLC plate (stationary phase)? 2. Why will some substances in a mixture travel farther up the TLC plate than others?
A student calculated the R(f) value for a spot to be 1.05. Why is the value...
A student calculated the R(f) value for a spot to be 1.05. Why is the value wrong? The progress of a reaction can be followed using TLC as follows: Several TLC plates are prepared by spotting them with the starting compound(s) and the product(s). (each plate is spotted with all the compounds,each in its own spot.) A place on each plate it saved for the reaction mixture.Periodically as the reaction proceeds (for example, every 30 minutes) a prepared plate is...
In 100 words or more, Why is it important to make sure you have a balanced...
In 100 words or more, Why is it important to make sure you have a balanced team? What can happen if you have too many people with the same strengths or weaknesses?
2. Why is logistics important on a macro level, and what contributions does logistics make in...
2. Why is logistics important on a macro level, and what contributions does logistics make in the economy?
Why is it important to know your needs and have documentation when you make requests of...
Why is it important to know your needs and have documentation when you make requests of superiors? Explain in your own words.
IMPORTANT: If you're going to write please make sure your writing is neat, legible, and easy...
IMPORTANT: If you're going to write please make sure your writing is neat, legible, and easy to read. Please write in print. Do not write in cursive. Thank you QUESTION: Explain when you would use an F-test rather than a T-test in econometrics
10. A shoreline observation post is located on a cliff such that the observer is 310 feet above sea level. When initially spotted, the angle of depression (the angle at which an observer needs to loo...
10. A shoreline observation post is located on a cliff such that the observer is 310 feet above sea level. When initially spotted, the angle of depression (the angle at which an observer needs to look down) from the observation post to an approaching ship was 4.8° a. Draw a diagram of the situation and label each known and unknown quantity measure b. How far out to sea was the ship when it was first spotted? c. After watching the...
I need help with my pre and post lab questions.
The three compounds below were spotted on a TLC plate and developed to give the TLC plate shown. Which compound corresponds to which spot? Calculate the Rf value for spot B in the TLC plate shown above. When using polarity to determine the relative rates at which organic compounds travel up the TLC plate, we are considering compounds of similar molecular weight. It should also be noted that Rf values...
Classify and breifky explain the different patterns of genetic
inheritance in humans
Questions for the Thin Layer Chromatography Laboratory 1. Why can't you use a pen to mark the origin of a TLC plate? Be explicit in your answer. 2. You were impatient to see how long it would take for your sample to resolve on the TLC plate, so you constantly removed the top from the chamber. Please explain why this is an incorrect practice. 3. What happens when...
1. When separating mixtures using TLC, why is it important to choose a solvent (mobile phase) that has a different polarity than the TLC plate (stationary phase)? 2. Why will some substances in a mixture travel farther up the TLC plate than others?
2. Why is logistics important on a macro level, and what contributions does logistics make in the economy?
10. A shoreline observation post is located on a cliff such that the observer is 310 feet above sea level. When initially spotted, the angle of depression (the angle at which an observer needs to look down) from the observation post to an approaching ship was 4.8° a. Draw a diagram of the situation and label each known and unknown quantity measure b. How far out to sea was the ship when it was first spotted? c. After watching the...
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