I have a lab report due on the chromatography of leaves, and the solvent used was...
Homework Answers
the Rf value of a particular pure dye or analyte in a particular solvent (or mixture) is constant if the following experimental conditions are kept unaltered:
- Temperature
- Chromatography medium, ie same type and grade of Chromatography Paper
- Solvent concentration and purity
- Amount of sample spotted on Chromatography medium
If Rf value is different from expected, then any 1 of above 4 can be reason
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I
have a lab report due on the chromatography of leaves, and the
solvent used was...
Post lab questions for chromatography and melting point lab. i have a few ideas of what the answers to these questions s...
Post lab questions for chromatography and melting point lab. i
have a few ideas of what the answers to these questions should be,
but I would like to double check. if anyone can help me, that would
be so awesome. Thank you!
A student cools a sample in a capillary tube after melting it. Upon attempting to determine the melting point on the same sample, the student does not obtain the same melting point. Why? A student places his TLC...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column /...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column / planar) The first step consists of the extraction of drug metabolites from biological fluids when we place the sample in a test tube containing a mixture of solvents and buffering salts that cause the extraction of basic and neutral drugs. The solvent extract of the sample is concentrated by heat and evaporation, then is deposited onto a small disc of chromatographic media (small circle...
Hi can someone help me with my pre lab theory questions (1,2,3) on rate of elution...
Hi can someone help me with my pre lab theory questions (1,2,3)
on rate of elution or Rf? Here is some background below. Please
help explain as many as possible (1-3)! I dont really get the
relation ships in this lab, thank you!
1. What factors affect the rate of elution in organic compounds? 2. Explain what is the relationship between polarities of compounds (polar/non- polar compound) and rate of elution (Rf). 3. Explain what is the relationship between solvent...
I need help with my pre and post lab questions. The three compounds below were spotted...
I need help with my pre and post lab questions.
The three compounds below were spotted on a TLC plate and developed to give the TLC plate shown. Which compound corresponds to which spot? Calculate the Rf value for spot B in the TLC plate shown above. When using polarity to determine the relative rates at which organic compounds travel up the TLC plate, we are considering compounds of similar molecular weight. It should also be noted that Rf values...
Chromatography I Questions Name CHE 201 Date The following solvent systems were found to separate compounds...
Chromatography I Questions Name CHE 201 Date The following solvent systems were found to separate compounds A and B by column chromatography: • A: hexane/ethyl acetate 10:1 • B: hexane/ethyl acetate 10:5 Which is the more polar compound, A or B? Explain your answer for credit, 2) In order to separate a mixture of A and B as in problem (1) by column chromatography: a. Which solvent system must be used first? Why? b. Which compound will elute from the...
LOS SCULUI EXPERIMENT # 6 PAPER CHROMATOGRAPHY LABORATORY REPORT DATA AND CALCULATIONS Measure the retention factors...
LOS SCULUI EXPERIMENT # 6 PAPER CHROMATOGRAPHY LABORATORY REPORT DATA AND CALCULATIONS Measure the retention factors (R) for each of the components and report the values in the data table. Student Name Course Section Date Instructor PRE-LAB ASSIGNMENT son Rd? Hetardation factor Re Value) is the distance a given component (analute) divided by the distance the Re values o f mobile phase There is no shry unit for i migration distance of substance Imigrahon 1. What are the units on...
Lab Day 8: Chromatography Prelab Questions 1) The basic food colors you can buy are red,...
Lab Day 8: Chromatography Prelab Questions 1) The basic food colors you can buy are red, blue areen, and yellow. Think about what you know about colors and predict which of these might be a single colored compound and which ones might contain more than one. write down your prediction. If you have the colors listed and want a purple color, what would you do? 2) Put the following chromatography solvents in order of polarity as a chromatography solvent: acetone,...
Bio 121 I need to make (yeast fermentation) lab report. This is the lab results: This...
Bio 121
I need to make (yeast fermentation) lab
report.
This is the lab results:
This is a guide to making the lab report:
Data Summary Sheet Date Experiment One: Glucose Fermentation Table One CO2 Production and Glucose Utilization over Time Actual Clock Time 7. 30 Elapsed Time in minutes ml. CO2 Glucose Concentration > 2000 9 ldl 10 3 m ymt 4. Som 50 m There will be two lines on the graph of the data from Tube #1...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography?...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
Im writing a lab report and have done a ttest but dont know how to report...
Im writing a lab report and have done a ttest but dont know how to report the information. it is a two tailed t test with a critical value of p=0.05.I got the mean of 1.07 s.d 0.3 and 2.64 s.d 0.77 n=6 with a t test value from excel of 0.004. how would I write this 1.07±0.3 ml/min for sample 1 and 2.64 ±0.77 ml/min sample 2 n=6 with paired t-test = 0.004 ,p value p˂0.05 is this right
Post lab questions for chromatography and melting point lab. i
have a few ideas of what the answers to these questions should be,
but I would like to double check. if anyone can help me, that would
be so awesome. Thank you!
A student cools a sample in a capillary tube after melting it. Upon attempting to determine the melting point on the same sample, the student does not obtain the same melting point. Why? A student places his TLC...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column / planar) The first step consists of the extraction of drug metabolites from biological fluids when we place the sample in a test tube containing a mixture of solvents and buffering salts that cause the extraction of basic and neutral drugs. The solvent extract of the sample is concentrated by heat and evaporation, then is deposited onto a small disc of chromatographic media (small circle...
Hi can someone help me with my pre lab theory questions (1,2,3)
on rate of elution or Rf? Here is some background below. Please
help explain as many as possible (1-3)! I dont really get the
relation ships in this lab, thank you!
1. What factors affect the rate of elution in organic compounds? 2. Explain what is the relationship between polarities of compounds (polar/non- polar compound) and rate of elution (Rf). 3. Explain what is the relationship between solvent...
I need help with my pre and post lab questions.
The three compounds below were spotted on a TLC plate and developed to give the TLC plate shown. Which compound corresponds to which spot? Calculate the Rf value for spot B in the TLC plate shown above. When using polarity to determine the relative rates at which organic compounds travel up the TLC plate, we are considering compounds of similar molecular weight. It should also be noted that Rf values...
Chromatography I Questions Name CHE 201 Date The following solvent systems were found to separate compounds A and B by column chromatography: • A: hexane/ethyl acetate 10:1 • B: hexane/ethyl acetate 10:5 Which is the more polar compound, A or B? Explain your answer for credit, 2) In order to separate a mixture of A and B as in problem (1) by column chromatography: a. Which solvent system must be used first? Why? b. Which compound will elute from the...
LOS SCULUI EXPERIMENT # 6 PAPER CHROMATOGRAPHY LABORATORY REPORT DATA AND CALCULATIONS Measure the retention factors (R) for each of the components and report the values in the data table. Student Name Course Section Date Instructor PRE-LAB ASSIGNMENT son Rd? Hetardation factor Re Value) is the distance a given component (analute) divided by the distance the Re values o f mobile phase There is no shry unit for i migration distance of substance Imigrahon 1. What are the units on...
Lab Day 8: Chromatography Prelab Questions 1) The basic food colors you can buy are red, blue areen, and yellow. Think about what you know about colors and predict which of these might be a single colored compound and which ones might contain more than one. write down your prediction. If you have the colors listed and want a purple color, what would you do? 2) Put the following chromatography solvents in order of polarity as a chromatography solvent: acetone,...
Bio 121
I need to make (yeast fermentation) lab
report.
This is the lab results:
This is a guide to making the lab report:
Data Summary Sheet Date Experiment One: Glucose Fermentation Table One CO2 Production and Glucose Utilization over Time Actual Clock Time 7. 30 Elapsed Time in minutes ml. CO2 Glucose Concentration > 2000 9 ldl 10 3 m ymt 4. Som 50 m There will be two lines on the graph of the data from Tube #1...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
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