Bovine serum albumin (BSA) is a biochemically useful protein. A 0.257 gram sample of bovine serum albumin is dissolved in water to make 0.146 L of solution, and the osmotic pressure of the solution at 25 °C is found to be 0.658 mbar. Calculate the molecular mass of bovine serum albumin.
the osmotic pressure, (pi), using the following equation:
Where:
= 0.658 × 10-3 = 0.000658 bar
M is the molar concentration of dissolved species (units of mol/L), M = Mass / Molar mass / Vol = 0.257/ M/ 0.146
R is the ideal gas constant, R = 0.08314 L bar
mol-1 K-1,
T is the temperature on the Kelvin scale, T = 298K
Molar mass of bovine serum albumin is 6628 g/mol
Bovine serum albumin (BSA) is a biochemically useful protein. A 0.257 gram sample of bovine serum...
Immunoglobulin G (IgG), formerly called gamma globulin, is a principal antibody in blood serum. A 0.677 g sample of immunoglobulin G is dissolved in water to make 0.127 L of solution, and the osmotic pressure of the solution at 25°C is found to be 0.881 mbar. Calculate the molecular mass of immunoglobulin G. molecular mass: g/mol
β-Galactosidase (β-gal) is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. A 0.360 gram sample of β-galactosidase is dissolved in water to make 0.108 L of solution, and the osmotic pressure of the solution at 25 °C is found to be 0.709 mbar. Calculate the molecular mass of β-galactosidase.
2. To study the binding of caffeine to bovine serum albumin by the equilibrium dialysis method, 2.8 x 10-4 M of albumin was allowed to equilibrate with 1 x 10-4 M of caffeine. After the equilibrium was established, 0.7 x 10-4M of caffeine was contained in the dialysis bag and 0.3 x 10-4 M of caffeine was found in the external solution. Calculate r, the ratio of bound to total protein, and B, the fraction of bound caffeine.
Calculate the molecular weight of a small protein if a 7.0-g sample of the protein dissolved in enough water to make 7.00 x 10 2 mL of solution has an osmotic pressure of 3.8 mmHg at 25.0°C.
The osmotic pressure of an aqueous solution of a certain protein was measured in order to determine the protein’s molar mass. The solution contained 3.50-g of protein dissolved in sufficient water to form 5.00-L of solution. The osmotic pressure of the solution at 25oC was found to be 1.54-mmHg. (Hint: Remember to convert mmHg to atm) Calculate the molar mass of the protein.
calculate the expected absorbace at 280 nm of a 1.5 mg/ml solution of bovine serum albumin based on the amino acid composition. all cysteins are involved in disulfide bonds. Calculate the expected A280 of a 1.50 mg/ml solution of bovine serum albumin, based on the amino acid composition (Table 1-1, page 28 from your manual). All the cysteines in bovine serum albumin are involved in disulfide bonds. T V W Y 10 9 0 6 12 11 0 7 6...
A 5.00-gram sample of a compound is dissolved in enough water to form 100.0 mL of solution. This solution has an osmotic pressure of 25 torr at 25
0.00100g of a certain protein is dissolved in 1.00ml of solution. The osmotic pressure of the solution is found to be 1.12 torr at 25° C. Calculate the molar mass of the protein. π = C x R x T x i
Using the regular solution model, show that oil and water do not mix unless the solution is very dilute. Assume oil is the solute. For hydrocarbon-water mixtures assume w-5 and T-300K. Do oil and water mix if x_av- 0.3? Calculate Delta G_MIX. Is it positive, negative, or zero? How about if x_mi = 0.0001? Do oil and water mix at this concentration of oil? At what mole fraction is the oil in equilibrium with its dissolved form? To solve this...
6. A 1.07 mg sample of a compound was dissolved in 78.1 mg of camphor. The resulting solution melted at 176.0 C. What is the molecular mass of the compound? The melting point of pure camphor is 179.5 C. The freezing-point-depression constant for camphor Kf is 40.0 C/m. 7. A solution is prepared by dissolving 5.88 g of an unknown nonelectrolyte in enough water to make 0.355 L of solution. The osmotic pressure of the solution is 1.21 atm at...