Question

Fish-liver oil is a good source of vitamin A, which is measured spectrophotometrically, after correcting for background, at a wavelength of 329 nm. When 0.1722 g of fish-liver oil is dissolved in 355 mL of solvent, the absorbance is 0.724 units. When 1.67 × 10⁻³ g of vitamin A is dissolved in 250. mL of solvent, the absorbance is 1.018 units. Calculate the vitamin A concentration in the fish-liver oil.

Answer 1