In E. coli translation of genes that contain a frameshift mutation often result in a truncated...
In E. coli translation of genes that contain a frameshift mutation often result in a truncated protein. This is because the frameshift mutation often result in a nonsense codon downstream in the DNA sequence. Can you come up with a reason why nonsense mutations are so prevalent after a shift in reading frame in an E. coli DNA sequence?
Homework Answers
Frame shift mutation used to shift the open reading frame of the nucleotide the sequence that the ribosomes used to read the codon frame shift mutation is due to the insertion of nucleotide sequence but not in a multiple of 3 . So after the frame shift it usually gets converted to nonsense codon which terminated the protein as a result it becomes truncated.
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In E. coli translation of genes that contain a frameshift
mutation often result in a truncated...
3. In E. coli translation of genes that contain a frameshift mutation often result in a...
3. In E. coli translation of genes that contain a frameshift mutation often result in a truncated protein. This is because the frameshift mutation often result in a nonsense codon downstream in the DNA sequence. Can you come up with a reason why nonsense mutations are so prevalent after a shift in reading frame in an E. coli DNA sequence? (1 marks)
Which of the following mutations could result in a truncated protein? Choose all that apply. A...
Which of the following mutations could result in a truncated protein? Choose all that apply. A missense mutation that changes a phenylalanine to a leucine O A two base pair frameshift O An in-frame removal of three base pairs O A one base pair frameshift A chromosomal-level deletion O o A chromosomal-level insertion O A nonsense mutation that changes a cysteine to a stop codon
The gene encoding protein Bog in E. coli has been changed by a point mutation in...
The gene encoding protein Bog in E. coli has been changed by a point mutation in its open reading frame. Originally the gene encoded a 108 amino acid long functioning protein whose amino acid composition favored nonpolar and negatively charged amino acids. After the mutation it encodes a 110 amino acid long non-functioning protein that is now rich in polar uncharged amino acids and positively charged amino acids. Which mutation scenario best explains this observation. A) A frame-shift caused by...
1) Which of the following mutations could result in a frameshift? A) a base insertion B)...
1) Which of the following mutations could result in a frameshift? A) a base insertion B) a base deletion C) a base substitution D) either A or B E) A, B, and C 2) Which of the following point mutations would be most likely to result in a non-functioning protein? A) a single base substitution in an intron B) a single base deletion near the end of the coding sequence C) a single base deletion in the codon following the...
What would the most likely phenotype of an E. coli colony containing pUC18 (without an insert...
What would the most likely phenotype of an E. coli colony containing pUC18 (without an insert fragment) be if the following de novo mutations occurred early in the growth of a single cell into a colony on your plate (assuming plate contains X-gal, IPTG and Amp)? a. A frameshift mutation in the fifth codon lacZ gene in pUC18. b. A loss of the pUC18 plasmid. c. A deletion of bps at positions 19-25 of the ampicillin resistance gene open reading...
O) The role of the 23S TRNA of the large ribosomal subunit of E. coli during...
O) The role of the 23S TRNA of the large ribosomal subunit of E. coli during protein synthesis is: a. aid in initiation of protein synthesis b. contributes to the process of translocation during the elongation stage of protein synthesis c. helps to form the A site within the ribosome d. actively forms a peptide bond e. none of the above P) Spontaneous mutations can be caused by: a. incorrect translation b. telomerase c. chemical mutagens d. tautomeric shifts e....
70. RNA synthesis in bacteria requires which of the following? a. DNA polymerase III b. A...
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13. Why are ribonucleoside triphosphates the monomers required for RNA synthesis rather than ribonucleoside monophosphates? A....
13. Why are ribonucleoside triphosphates the monomers required for RNA synthesis rather than ribonucleoside monophosphates? A. Only ribonucleoside triphosphates contain the sugar ribose. B. Ribonucleoside triphosphates have low potential energy, making the polymerization reaction endergonic. C. Ribonucleoside triphosphates have high potential energy, making the polymerization reaction exergonic. D. Ribonucleoside monophosphates cannot form complementary base pairs with the DNA template. E. Ribonucleoside triphosphates are not used, rather all use deoxyriboside triphosphates. 14. How is a mutation in a bacterial cell that...
3. Consider a hypothetical strain of E. coli (strain 401) that contains two mutations that affect...
3. Consider a hypothetical strain of E. coli (strain 401) that contains two mutations that affect tryptophan biosynthesis. The first mutation is a single nucleotide substitution that converts the second tandem Trp codon in region 1 of the Trp leader sequence into a stop codon (see slides 2-7 of Lecture 24 on iLearn) trp structural genes trpE trpD trpC trpB trpA PO Trp Leader Met-Lys-Ala-lle-Phe-Val-Leu-Lys-Gly-Trp-Trp-Arg-Thr Ser- Stop Assume that strain 401 also contains a mutation in the gene for the...
12. Nucleus 13. Point mutation 14. Deletion mutation 15. Exons 16. Translation 17. Nitrogenous bases H...
12. Nucleus 13. Point mutation 14. Deletion mutation 15. Exons 16. Translation 17. Nitrogenous bases H bonded 18. mRNA B. Enzyme that unwinds DNA double helix C. Sugar found in DNA nucleotide D. Process of making a protein E. Substitution of one nucleotide base pair for another F. Rungs (steps) of DNA "ladder" G. Transcription occurs in this part of the cell H. Enzyme that synthesizes DNA by connecting bases tha are complementary to the original template stra 1. Removing...
3. In E. coli translation of genes that contain a frameshift mutation often result in a truncated protein. This is because the frameshift mutation often result in a nonsense codon downstream in the DNA sequence. Can you come up with a reason why nonsense mutations are so prevalent after a shift in reading frame in an E. coli DNA sequence? (1 marks)
Which of the following mutations could result in a truncated protein? Choose all that apply. A missense mutation that changes a phenylalanine to a leucine O A two base pair frameshift O An in-frame removal of three base pairs O A one base pair frameshift A chromosomal-level deletion O o A chromosomal-level insertion O A nonsense mutation that changes a cysteine to a stop codon
O) The role of the 23S TRNA of the large ribosomal subunit of E. coli during protein synthesis is: a. aid in initiation of protein synthesis b. contributes to the process of translocation during the elongation stage of protein synthesis c. helps to form the A site within the ribosome d. actively forms a peptide bond e. none of the above P) Spontaneous mutations can be caused by: a. incorrect translation b. telomerase c. chemical mutagens d. tautomeric shifts e....
13. Why are ribonucleoside triphosphates the monomers required for RNA synthesis rather than ribonucleoside monophosphates? A. Only ribonucleoside triphosphates contain the sugar ribose. B. Ribonucleoside triphosphates have low potential energy, making the polymerization reaction endergonic. C. Ribonucleoside triphosphates have high potential energy, making the polymerization reaction exergonic. D. Ribonucleoside monophosphates cannot form complementary base pairs with the DNA template. E. Ribonucleoside triphosphates are not used, rather all use deoxyriboside triphosphates. 14. How is a mutation in a bacterial cell that...
3. Consider a hypothetical strain of E. coli (strain 401) that contains two mutations that affect tryptophan biosynthesis. The first mutation is a single nucleotide substitution that converts the second tandem Trp codon in region 1 of the Trp leader sequence into a stop codon (see slides 2-7 of Lecture 24 on iLearn) trp structural genes trpE trpD trpC trpB trpA PO Trp Leader Met-Lys-Ala-lle-Phe-Val-Leu-Lys-Gly-Trp-Trp-Arg-Thr Ser- Stop Assume that strain 401 also contains a mutation in the gene for the...
12. Nucleus 13. Point mutation 14. Deletion mutation 15. Exons 16. Translation 17. Nitrogenous bases H bonded 18. mRNA B. Enzyme that unwinds DNA double helix C. Sugar found in DNA nucleotide D. Process of making a protein E. Substitution of one nucleotide base pair for another F. Rungs (steps) of DNA "ladder" G. Transcription occurs in this part of the cell H. Enzyme that synthesizes DNA by connecting bases tha are complementary to the original template stra 1. Removing...
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