The total retention time of an analyte on a GC column was 5 min. The column efficiency according to this peak was 22,500 total theoretical plates. Find the width of the peak at the base.
The total retention time of an analyte on a GC column was 5 min. The column...
A GC column is operated under the following conditions: Column: 1.10 m x 2 mm packed with Chromosorb P and Carbowax 20M as stationary phase. The following retention times were obtained: air: 18.0 sec; methylacetate: 2.98 min; methyl propionate: 4.56 min. Peak width at half height: methylacetate: 7.7 sec; methyl propionate: 12.4 sec. Calculate: a) the corrected retention time for each solute. b) the capacity factor k' for each solute. c) the selectivity factor α for the pair of solutes....
Two compounds C and D are separated on a column 34 cm long . Given the chromatographic data below calculate The column resolution? , the number of theoretical plates ?the height of theoretical plate and what length of column would be required to give a resolution of 1.5? Compound C retention time 18.3 width at base of peak(min) = 2.18 Compound D retention time 17.7, width at base 1.23
A 20-m long GC column was operated at 80 °C. A small peak for air had a retention time of 419 s and a base peak width of 14.8 s. A peak for heptane had a retention time of 536 s and a base peak width of 19.4 s. What is the chromatographic resolution between the air and heptane peaks?
A solute with a retention time of 407s has a width at the base of 13.0s on a column 12.2m long. Find the number of plates and plate heigh. The half width of the same peak is 7.6s. Find the plate height
The table contains data for a liquid chromatography separation of four compounds, A, B, C, and D, on a 40.0 cm column Retention Compound time (min) Width at the base (min) A 5.17 0.436 B 1.02 13.0 15.0 C 1.10 23.4 1.91 Calculate the number of theoretical plates, N, for each peak. NA NB Nc ND = What is the average number of theoretical plates, Navg, for Navg the column? What is the plate height, H, for the column? Н-...
Retention Height Width 1/2 Peak Time (min) (mm) (min) A 1.82 1,922 0.036 B 3.63 1,369 0.071 C 7.69 1,282 0.090 The above separation was performed on a 80 m column. What is the average HETP value (in cm) calculated from the above GC separation data? Type your answer rounded to the 3rd decimal place without units.
A solute with a retention time of 4 mins 25 second has a base width of 12 seconds. The column is 30.0m long. How many theoretical plates does the column have? What is length in mm of one theoretical plate?
A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, is, was found to be 218.0 s with a baseline peak width, wc, of 14.4 s. The retention time for aspartame, 1., was 250.5 s with a baseline peak width, w of 21.1 s. The retention time for the unretained solvent methanol was 40.4 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 27.7...
A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, tc,was found to be 207.6 s with a baseline peak width, wc, of 16.2 s. The retention time for aspartame, ta, was 253.7 s with a baseline peak width, wa, of 18.7 s. The retention time for the unretained solvent methanol was 40.7 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 27.3 cmlong...
1. if you were to see a peak in your GC-MS sample with a retention time of 8.00 min, how would the polarity of that compound compare to the polarity of limonene? Explain. 2. what is the purpose of adding the anhydrous sodium sulfate to GC-MS vial and filtering it? what might happen during the GC-MS analysis if we didn’t perform these steps?