The one-letter sequence is: SHIRK a) What is the isoelectric point? b) What is the average...
The one-letter sequence is: SHIRK
a) What is the isoelectric point?
b) What is the average charge on the population of peptide macromolecules at pH = 6.0?
c) What is the average charge on the population of peptide macromolecules at pH = 9.2?
d) What is the average charge on the population of peptide macromolecules at pH = 10.5?
Homework Answers
a) Isoelectric point is the pH at which a particular molecule carries no net electrical charge or is electrically neutral in statistical mean.
b) Qpeptide, Qp= (+1)/(1+10(pH-8.5)) + (+2)/(1+10(pH-10.5)) + (-1)/(1+10-(pH-3.2)) + (-4)/(1+10-(pH-3.9)) ------(A)
use value of pH= 6.0 in above equation number A:
Therefore, Qp= -1.97 (at pH=6.0)
c) Qp at pH= 9.2
Use value of pH= 9.2 in equation A:
Qp= -2.93 (at pH= 9.2)
d) Qp at pH= 10.5
Use value of pH= 10.5 in equation A:
Qp= -2.99 (at pH= 10.5)
Add Answer to:
The one-letter sequence is: SHIRK
a) What is the isoelectric point?
b) What is the average...
The one-letter sequence is: WATER a) Draw the peptide (R-groups trans), indicating charges, in pr...
The one-letter sequence is: WATER
a) Draw the peptide (R-groups trans), indicating charges, in
predominant form found at pH = 0.
b) What is the isoelectric point?
c) What is the average charge on the population of peptide
macromolecules at pH = 2.2?
d) What is the average charge on the population of peptide
macromolecules at pH = 12.5?
TABLE 4.1 Amino Acid Alanine Arginine Asparagine Aspartic acid Cysteine....« Glutamic acid Glutamine Glycine Histidine Isoleucine Leucine Lysine … Methionine Phenylalanine...
The isoelectric point (pI) of a peptide is the pH at which the peptide does not...
The isoelectric point (pI) of a peptide is the pH at which the
peptide does not migrate in an electric field. Since the peptide is
zwitterionic, there are the same number of positive charges as
negative charges on the peptide population. The pI can be estimated
fairly accurately (within 0.1 or 0.2 pH units) from the pK values
of all the proton dissociable groups in the peptide. Using pK
values from the table at the right, estimate the pI value...
The isoelectric point (pI) of a peptide is the pH at which the peptide does not...
The isoelectric point (pI) of a peptide is the pH at which the
peptide does not migrate in an electric field. Since the peptide is
zwitterionic, there are the same number of positive charges as
negative charges on the peptide population. The pI can be estimated
fairly accurately (within 0.1 or 0.2 pH units) from the pK values
of all the proton dissociable groups in the peptide. Using pK
values from the table at the right, estimate the pI value...
6. A peptide has the sequence Cys-His-Phe-Glu-Ala-Arg a. Write the single letter sequence of the peptide...
6. A peptide has the sequence Cys-His-Phe-Glu-Ala-Arg a. Write the single letter sequence of the peptide b. Calculate the percentage and indicate the charge of the predominant peptide species at pH 6.5. Use the following pKa values: pKa Cys = 8.3, pKa N-terminus = 10.8, pKa His =6.0, pKa Glu = 4.3, pKa Arg = 12.5, pKa C-terminus =2.0 c. What is the charge of the peptide at pH 4.3? d. Draw the chemical structure of the predominant peptide species...
Peptides can be separated using an ion-exchange column based on their isoelectric (pl) values. At which...
Peptides can be separated using an ion-exchange column based on their isoelectric (pl) values. At which pH values would two different peptides, one with a pl of 5.0 and the other with a pl of 9.2, bind to a cation- and anion-exchange column? Each peptide may be capable of binding to each column at more than one pH value. anion-exchange column at pH = 3.0 cation-exchange column at pH = 3.0 Answer Bank peptide B pI = 9.2 peptide A...
The isoelectric point (pI) of a peptide is the pH at which the peptide does not...
The isoelectric point (pI) of a peptide is the pH at which the peptide does not migrate in an electric field. Since the peptide is zwitterionic, there are the same number of positive charges as negative charges on the peptide population. The pI can be estimated fairly accurately (within 0.1 or 0.2 pH units) from the pK values of all the proton dissociable groups in the peptide. Using pK values from the table at the right, estimate the pI value...
What is the approximate isoelectric point of the peptide: Methionine-Histidine-Threonine-Aspartate pKa’s: Methionine: 2.3, 9.2 Histidine: 1.8,...
What is the approximate isoelectric point of the peptide: Methionine-Histidine-Threonine-Aspartate pKa’s: Methionine: 2.3, 9.2 Histidine: 1.8, 9.3, 6.0 (R-group) Threonine: 2.1, 9.6 Aspartate: 1.9, 9.6, 3.7 (R-group) Group of answer choices 9.4 2.8 7.6 4.8 1.6
Please be clear with how you determined the code sequence and isoelectric point 7. A single...
Please be clear with how you determined the code sequence and
isoelectric point
7. A single 14 amino acid peptide chain is to be assigned from the following experimental information below, your job is to provide the full triple letter code sequence (if possible), calculate its isoelectrie point and show your logie behind your answer. (12 marks) Experimental information: (i) Amino acid analysis: Cx2, Exl, Fxl, Wx1, Gx1, Hx1, Axl, Lx1, Dx2, Rx1, Qxl, and Nxl. (ii) Chymotrypsin Digestion of...
The isoelectric point, pl, of the protein trypsin is 10.5, while that of clostripain is 4.8...
The isoelectric point, pl, of the protein trypsin is 10.5, while that of clostripain is 4.8 . What is the net charge of trypsin at pH 5.1 ? What is the net charge of clostripain at pH 2.5 ?! The isoelectric point of cysteine is 5.07 ; histidine , 7.59. During paper electrophoresis at pH 4.3 , toward which electrode does cysteine migrate? During paper electrophoresis at pH 7.1 , toward which electrode does histidine migrate?
What is the approximate net charge of the following pentapeptide at pH 10? Arg-Gln-Cys-His-Ala What is...
What is the approximate net charge of the following pentapeptide at pH 10? Arg-Gln-Cys-His-Ala What is the Isoelectric point (pI) of the peptide given above? Use the pKa values given here when needed: Side group/ amino acid pKa Asp 3.9 Glu 4.1 HIs 6.0 Cys 8.4 Tyr 10.5 Lys 10.5 Arg 12.5 C-term 3.5 N-term 9.0
The one-letter sequence is: WATER
a) Draw the peptide (R-groups trans), indicating charges, in
predominant form found at pH = 0.
b) What is the isoelectric point?
c) What is the average charge on the population of peptide
macromolecules at pH = 2.2?
d) What is the average charge on the population of peptide
macromolecules at pH = 12.5?
TABLE 4.1 Amino Acid Alanine Arginine Asparagine Aspartic acid Cysteine....« Glutamic acid Glutamine Glycine Histidine Isoleucine Leucine Lysine … Methionine Phenylalanine...
The isoelectric point (pI) of a peptide is the pH at which the
peptide does not migrate in an electric field. Since the peptide is
zwitterionic, there are the same number of positive charges as
negative charges on the peptide population. The pI can be estimated
fairly accurately (within 0.1 or 0.2 pH units) from the pK values
of all the proton dissociable groups in the peptide. Using pK
values from the table at the right, estimate the pI value...
The isoelectric point (pI) of a peptide is the pH at which the
peptide does not migrate in an electric field. Since the peptide is
zwitterionic, there are the same number of positive charges as
negative charges on the peptide population. The pI can be estimated
fairly accurately (within 0.1 or 0.2 pH units) from the pK values
of all the proton dissociable groups in the peptide. Using pK
values from the table at the right, estimate the pI value...
6. A peptide has the sequence Cys-His-Phe-Glu-Ala-Arg a. Write the single letter sequence of the peptide b. Calculate the percentage and indicate the charge of the predominant peptide species at pH 6.5. Use the following pKa values: pKa Cys = 8.3, pKa N-terminus = 10.8, pKa His =6.0, pKa Glu = 4.3, pKa Arg = 12.5, pKa C-terminus =2.0 c. What is the charge of the peptide at pH 4.3? d. Draw the chemical structure of the predominant peptide species...
Peptides can be separated using an ion-exchange column based on their isoelectric (pl) values. At which pH values would two different peptides, one with a pl of 5.0 and the other with a pl of 9.2, bind to a cation- and anion-exchange column? Each peptide may be capable of binding to each column at more than one pH value. anion-exchange column at pH = 3.0 cation-exchange column at pH = 3.0 Answer Bank peptide B pI = 9.2 peptide A...
Please be clear with how you determined the code sequence and
isoelectric point
7. A single 14 amino acid peptide chain is to be assigned from the following experimental information below, your job is to provide the full triple letter code sequence (if possible), calculate its isoelectrie point and show your logie behind your answer. (12 marks) Experimental information: (i) Amino acid analysis: Cx2, Exl, Fxl, Wx1, Gx1, Hx1, Axl, Lx1, Dx2, Rx1, Qxl, and Nxl. (ii) Chymotrypsin Digestion of...
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