What is the approximate isoelectric point of the peptide: Methionine-Histidine-Threonine-Aspartate pKa’s: Methionine: 2.3, 9.2 Histidine: 1.8,...
What is the approximate isoelectric point of the peptide:
Methionine-Histidine-Threonine-Aspartate
pKa’s:
Methionine: 2.3, 9.2
Histidine: 1.8, 9.3, 6.0 (R-group)
Threonine: 2.1, 9.6
Aspartate: 1.9, 9.6, 3.7 (R-group)
Group of answer choices
9.4
2.8
7.6
4.8
1.6
Homework Answers
Isoelectric pH means pH at which the peptide will have no net charge .
It will be pH 4.8 because at this pH pka of acidic group less than 4.8 will be negative by releasing H+ and pka more than it will be positive by accepting H+.
And here N terminal amino acid methionine has pka 2.3 and aspartate c terminal pka is 9.6.so pH will be in between them so that one POSITIVE and one negative charge and thus net charge becomes zero.
Here is two side chain one is amino present in histidine and one in acidic in aspartate.
So pH should also be in between them to make one acidic negative charge and one amino group positive charge .
So the pH should be in between 3 u and 6 which is 4.8.
Thank you
Add Answer to:
What is the approximate isoelectric point of the peptide:
Methionine-Histidine-Threonine-Aspartate
pKa’s:
Methionine: 2.3, 9.2
Histidine: 1.8,...
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INN MARGIN ROOMS NEAREST OFFICE COLLEGE INCOME DISTTWN 1 44.2 3471 2.1 523 12 35 9.4...
INN
MARGIN
ROOMS
NEAREST
OFFICE
COLLEGE
INCOME
DISTTWN
1
44.2
3471
2.1
523
12
35
9.4
2
29.8
3567
1.8
140
13.5
42
5.7
3
38.4
3264
1.6
404
22.5
45
4.4
4
54.4
3234
1.1
649
19.5
35
6.5
5
34.5
2730
4
171
17
41
10.5
6
44.9
3003
3.4
402
15.5
37
4.6
7
46
2341
2
580
23
45
7.4
8
50.2
3021
2.6
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33
9.3
9
46
2655
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22
40
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the response variable, and MALE (x1), BIRTH (x2), DIVO (x3), BEDS
(x4), EDUC (x5), and INCO (x6), as predictors.
"STATE" "MALE" "BIRTH" "DIVO" "BEDS" "EDUC" "INCO" "LIFE"
AK 119.1 24.8 5.6 603.3 14.1 4638 69.31
AL 93.3 19.4 4.4 840.9 7.8 2892 69.05
AR 94.1 18.5 4.8 569.6 6.7 2791 70.66
AZ 96.8 21.2 7.2 536.0 12.6 3614 70.55
CA 96.8 18.2 5.7 649.5 13.4 4423 71.71
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Data file: "STATE" "MALE" "BIRTH" "DIVO" "BEDS" "EDUC" "INCO" "LIFE" AK 119.1 24.8 5.6 603.3 14.1...
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INN
MARGIN
ROOMS
NEAREST
OFFICE
COLLEGE
INCOME
DISTTWN
1
44.2
3471
2.1
523
12
35
9.4
2
29.8
3567
1.8
140
13.5
42
5.7
3
38.4
3264
1.6
404
22.5
45
4.4
4
54.4
3234
1.1
649
19.5
35
6.5
5
34.5
2730
4
171
17
41
10.5
6
44.9
3003
3.4
402
15.5
37
4.6
7
46
2341
2
580
23
45
7.4
8
50.2
3021
2.6
572
8.5
33
9.3
9
46
2655
3.2
666
22
40
6.7...
We consider a multiple linear regression model with LIFE (y) as
the response variable, and MALE (x1), BIRTH (x2), DIVO (x3), BEDS
(x4), EDUC (x5), and INCO (x6), as predictors.
"STATE" "MALE" "BIRTH" "DIVO" "BEDS" "EDUC" "INCO" "LIFE"
AK 119.1 24.8 5.6 603.3 14.1 4638 69.31
AL 93.3 19.4 4.4 840.9 7.8 2892 69.05
AR 94.1 18.5 4.8 569.6 6.7 2791 70.66
AZ 96.8 21.2 7.2 536.0 12.6 3614 70.55
CA 96.8 18.2 5.7 649.5 13.4 4423 71.71
CO 97.5...
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