You will construct a Lineweaver-Burk diagram, which uses 1/[S] as the independent variable. The substrate used...
- You will construct a Lineweaver-Burk diagram, which uses 1/[S] as the independent variable. The substrate used will be (S)-2-Amino-3-(3,4-dihydroxyphenyl)propanoic acid, also called L-3,4-dihydroxyphenylalanine or L-dopa. Its molecular mass is 197.1879 g/mole. We will prepare a stock solution of 4 mg/mL. What molarity in mM does this correspond to? (1 pt) Assume you are going to preparing 5 cuvettes, each containing a total volume of 5 mL. The cuvettes will contain different amounts of the L-dopa stock solution: 0.1 mL, 0.15 mL, 0.2 mL, 0.25 and 0.3 mL. Calculate [S] and 1/[S] with [S] in mM for all cuvettes! (5 pts) In the lab, you will use the 1/[S] values for constructing the Lineweaver-Burk diagram.
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You will construct a Lineweaver-Burk diagram, which uses 1/[S]
as the independent variable. The substrate used...
how do you make a lineweaver-burk plot where the trend line extends backwards? is there a...
how do you make a lineweaver-burk plot where the trend line
extends backwards? is there a way of figuring out how far back it
should extend based of your data ? As well, how do you plot two
sets of data on one graph ? Thank you! (idk if you need to see my
data-> attached below)
Biochemistry Enzyme Kinetics Assignment Four answers for this assignment will be completed in elearn: En in elearn: Enzyme Kinetics Quiz ots but must...
Trial 6 Trial 5 Trial 2 Trial 4 Trial 3 Trial 1 Volume of acid sml...
Trial 6 Trial 5 Trial 2 Trial 4 Trial 3 Trial 1 Volume of acid sml solution acquired: smit smi smi 6 мм ISmI 1 omi omi omlomi Initial buret reading: OML Oml Final buret reading: umu 5ml smi u.sms . s u .s. *Volume of NaOH I UML solution used: smi I smi u.5ml 9.5 u.s. I volume trail m NaOH t (trail) 7 mol NaOH MGIHAI > MHCL *Molarity of acid solution: Name: *Average Molarity of acid: Percent...
DATA TO BE USED Please use the data provided below to complete this assignment. Refer to...
DATA TO BE USED Please use the data provided below to complete this assignment. Refer to Expt. 7 in the laboratory manual for an explanation of the data. o Mass used to prepare 5.0 g/L sodium sulfate solution: 0.494 g o Mass used to prepare 0.025 M sodium sulfate solution: 0.351 g o Mass used to prepare 2.0 mM sodium sulfate solution: 0.025 g o Conductivity (in uS/cm) for 5.0 g/L and 0.025 M and 2.0 mM sodium sulfate solutions...
I only need help with part D. The answer is 0.065 sec-1, but I need help...
I only need help with part D. The answer is
0.065 sec-1, but I need help to understand why.
You are studying a dehydrogenase enzyme that catalyzes the formation of NADH from NAD^+. Subsequent studies determined that NADU will absorb at 340 nm while NAD^+ will not. The extinction coefficient for NADH is 6220 M^-1 cm^-1. The reaction volume is 750 mu L total and the substrate molecular weight is 223 g/mol. You used 20 mu L of enzyme from...
3. Use the below tables to complete lab calculations on the worksheet on pages 2-6 of...
3. Use the below tables to complete lab calculations on the worksheet on pages 2-6 of this document. You will submit the worksheet via dropbox on Canvas by you assigned lab time the week of March 30th through April 3rd. A document with sample calculations of different concentrations is provided to you on canvas. Enzyme Kinetics Lab Buffer volume (mL) Enzyme Volume (ml) Substrate Volume (ml) TOTAL VOLUME (mL) 0.04 0.5 1.5 0.04 0.25 1.5 0.04 0.1 1.5 0.04 0.05...
Determine how many liters (l) of stock HCl were used in titration #1. Please fill all...
Determine how many liters (l) of stock HCl were used
in titration #1.
Please fill all questions under "Data Analysis"
Page 1 is just instruction for Page 2
EXPT 10: TITRATION OF STRONG ACID WITH STRONG BASE Note to Students: when you see the symbol (), this means there is something you must add into the mock data yourself. You may handwrite or type your answers directly on these pages for submission to a Blackboard link. Remember to show all...
I need help with finding the total added SCN concentration and Total added Fe3+. If I can see two examples of each, then I will be able to find the rest on my own. Data Tab Molarity of stock KSCN sol...
I need help with finding the total added SCN concentration and
Total added Fe3+. If I can see two examples of each, then I will be
able to find the rest on my own.
Data Tab Molarity of stock KSCN solution Molarity of stock Fe(NOs)s solutiona Total added Total added Absorbance SCN cone Few cone at 447 nm Test mL Stock l Stoek Tube mL. H,o KSCN Fe(NO,)s M) 4.0 2 S dlo .2.S (o3 2. 1I. Data Collection- Quantitative:...
1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2....
1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2. In part one of the experiment, you will prepare the acid solutions being titrated from a stock solution. Describe how you will accurately prepare 10.00 mL of 0.100 M HCl solution using a 1.00 M HCl stock solution. In your response to this question, be very specific about the quantities of stock solution and deionized water to be used in the dilution and the...
please help with my pre lab additional information Pre-Lab Questions: 1. What is the definition of...
please help with my pre lab
additional information
Pre-Lab Questions: 1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2. In part one of the experiment, you will prepare the acid solutions being titrated from a stock solution. Describe how you will accurately prepare 10.00 mL of 0.100 M HCl solution using a 1.00 M HCI stock solution. In your response to this question, be very specific about the quantities of stock solution and...
Problems 1. According to the Ideal Gas Law, which of the following is/are correct for a...
Problems 1. According to the Ideal Gas Law, which of the following is/are correct for a gas cylinder of fixed volume filled with one mole of oxygen gas? Please explain each one. a. When the temperature of the cylinder changes from 15 °C to 30 °C, the pressure inside the cylinder doubles. b. When a second mole of oxygen is added to the cylinder, the ratio T/P remains constant. C. An identical cylinder filled with the same pressure of hydrogen...
how do you make a lineweaver-burk plot where the trend line
extends backwards? is there a way of figuring out how far back it
should extend based of your data ? As well, how do you plot two
sets of data on one graph ? Thank you! (idk if you need to see my
data-> attached below)
Biochemistry Enzyme Kinetics Assignment Four answers for this assignment will be completed in elearn: En in elearn: Enzyme Kinetics Quiz ots but must...
Trial 6 Trial 5 Trial 2 Trial 4 Trial 3 Trial 1 Volume of acid sml solution acquired: smit smi smi 6 мм ISmI 1 omi omi omlomi Initial buret reading: OML Oml Final buret reading: umu 5ml smi u.sms . s u .s. *Volume of NaOH I UML solution used: smi I smi u.5ml 9.5 u.s. I volume trail m NaOH t (trail) 7 mol NaOH MGIHAI > MHCL *Molarity of acid solution: Name: *Average Molarity of acid: Percent...
DATA TO BE USED Please use the data provided below to complete this assignment. Refer to Expt. 7 in the laboratory manual for an explanation of the data. o Mass used to prepare 5.0 g/L sodium sulfate solution: 0.494 g o Mass used to prepare 0.025 M sodium sulfate solution: 0.351 g o Mass used to prepare 2.0 mM sodium sulfate solution: 0.025 g o Conductivity (in uS/cm) for 5.0 g/L and 0.025 M and 2.0 mM sodium sulfate solutions...
I only need help with part D. The answer is
0.065 sec-1, but I need help to understand why.
You are studying a dehydrogenase enzyme that catalyzes the formation of NADH from NAD^+. Subsequent studies determined that NADU will absorb at 340 nm while NAD^+ will not. The extinction coefficient for NADH is 6220 M^-1 cm^-1. The reaction volume is 750 mu L total and the substrate molecular weight is 223 g/mol. You used 20 mu L of enzyme from...
3. Use the below tables to complete lab calculations on the worksheet on pages 2-6 of this document. You will submit the worksheet via dropbox on Canvas by you assigned lab time the week of March 30th through April 3rd. A document with sample calculations of different concentrations is provided to you on canvas. Enzyme Kinetics Lab Buffer volume (mL) Enzyme Volume (ml) Substrate Volume (ml) TOTAL VOLUME (mL) 0.04 0.5 1.5 0.04 0.25 1.5 0.04 0.1 1.5 0.04 0.05...
Determine how many liters (l) of stock HCl were used
in titration #1.
Please fill all questions under "Data Analysis"
Page 1 is just instruction for Page 2
EXPT 10: TITRATION OF STRONG ACID WITH STRONG BASE Note to Students: when you see the symbol (), this means there is something you must add into the mock data yourself. You may handwrite or type your answers directly on these pages for submission to a Blackboard link. Remember to show all...
I need help with finding the total added SCN concentration and
Total added Fe3+. If I can see two examples of each, then I will be
able to find the rest on my own.
Data Tab Molarity of stock KSCN solution Molarity of stock Fe(NOs)s solutiona Total added Total added Absorbance SCN cone Few cone at 447 nm Test mL Stock l Stoek Tube mL. H,o KSCN Fe(NO,)s M) 4.0 2 S dlo .2.S (o3 2. 1I. Data Collection- Quantitative:...
1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2. In part one of the experiment, you will prepare the acid solutions being titrated from a stock solution. Describe how you will accurately prepare 10.00 mL of 0.100 M HCl solution using a 1.00 M HCl stock solution. In your response to this question, be very specific about the quantities of stock solution and deionized water to be used in the dilution and the...
please help with my pre lab
additional information
Pre-Lab Questions: 1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2. In part one of the experiment, you will prepare the acid solutions being titrated from a stock solution. Describe how you will accurately prepare 10.00 mL of 0.100 M HCl solution using a 1.00 M HCI stock solution. In your response to this question, be very specific about the quantities of stock solution and...
Problems 1. According to the Ideal Gas Law, which of the following is/are correct for a gas cylinder of fixed volume filled with one mole of oxygen gas? Please explain each one. a. When the temperature of the cylinder changes from 15 °C to 30 °C, the pressure inside the cylinder doubles. b. When a second mole of oxygen is added to the cylinder, the ratio T/P remains constant. C. An identical cylinder filled with the same pressure of hydrogen...
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