Question

how do you make a lineweaver-burk plot where the trend line extends backwards? is there a way of figuring out how far back it should extend based of your data ? As well, how do you plot two sets of data on one graph ? Thank you! (idk if you need to see my data-> attached below)

Biochemistry Enzyme Kinetics Assignment Four answers for this assignment will be completed in elearn: En in elearn: Enzyme Kinetics Quiz ots but must hand in a hand plotted tou may use excel to do the lineweaver-Burk plots but must hd graph into the dropbox. Note: If your graph is not handed in your assignment grade will your assignment grade will become zero. around that catalyzes the reaction between students, S and academic An enzyme was found that catalyzes th success, P (for pass); the enzyme was called studyase. An enzyme assay was run on Stud Da = 1g/mol) was placed in a set of test tubes each of which containe substrate student at varying concentrations success (P) was determined. ay was run on studyase. 10ue of studyase, molecular weight 20,000 Set of test tubes each of which contained 1mL of the we student at varying concentrations and the amount of product, academic The following results were determined: s, (mm) Velocity, V (mmole/mL/sec) 30 2.50 5.00 10.00 20.00 30.00 50 70 85 100 a. Plot a Lineweaver-Burk plot of 1/V versus 1/S. Answer Question 1. b. Determine Vmax and km for the enzyme studyase. Answer Questions 283 Calculate kcat and the catalytic constant (kcat/km) Tor the enzyme studvase find (E] as total mmoles/mL). Answer Question 4&5 d. Another enzyme called partyase was analyzed for this rea mmoles/mL of this enzyme was assayed it was found to hav, esaved it was found to have a Vmax of 65.10 as a higher affinity for the substrate? Answ mmol/ml/sec and a Km of 10.00 mm. Calculate Kcat for partyase. Answer Question 6 e. Which enzyme studyase or partyase has a h has a higher kinetic efficiency? Answer Question 7 Which enzyme studyase or partyase has a

Answer 1

Just extrapolate your graph with the help of scale and you will get to know how far the line will go backward. I have attached the graph for your reference.

아 0U 00033 0.02 Oool4 0002 이와 002 0 11 0.05 Oo3 0여 603 Slope = Km_ slope 싸자 002 /Nmax 이 005 0 OS | 이 이 OIS 0 -2 25 003 0035 00y