Question

Please fill out the missing sections, either using the drop-down options or by filling in boxes (all boxes require numerical answers). Prior to next generation sequencing, the most commonly used DNA sequencing method was Sanger, or (______) sequencing. Sanger sequencing comes in two forms, manual and automated. One of the main differences between these methods (apart from one is manual and one is semi-automated) is the means by which reaction products are detected. In manual sequencing reaction products are detected by (____), whereas in automated sequencing reaction products are detected by (_____)

Answer 1

The most commonly used DNA sequencing method was Sanger sequencing also known as the chain termination method sequencing. Sanger sequencing comes in two forms, manual and automated. One of the big difference between these method is the means by which reaction products are detected. In manual sequencing reaction products are detected by using dideoxy nucleotides and in the automated sequencing reaction products are detected by fluorescent labelling or autoradiography.