The specific activity of a pure enzyme is 6000 μmol substrate transformed.min-1.(mg protein)-1 and its molecular...
The specific activity of a pure enzyme is 6000 μmol substrate
transformed.min-1.(mg protein)-1 and its molecular weight is 10,000
Da. Calculate the turnover number of the enzyme in units of μmol
substrate transformed.(μmol enzyme)-1.sec-1]? Show all workings
including units.
Note: A calculator should not be required to answer this
question.
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The specific activity of a pure enzyme is 6000 μmol substrate
transformed.min-1.(mg protein)-1 and its molecular...
included proper units when HLLL 1. The following kinetic data were generated for the enzyme SpB1,...
included proper units when HLLL 1. The following kinetic data were generated for the enzyme SpB1, this enzyme has an unknown biological function. Using the information provided below answer questions la-le. All reactions had 0.1 mg/mL SPBT which based on amino acid sequence and gel filtration column chromatography has a molecular weight of 42,500 Daltons. S002- Substrate 1 Substrate 2 KM (MM) kcat (sec) glucose >50 <0.01 glucose NADP 4+1 0.5+0.07 glucose NAD 2718 2 +0.05 galactose >50 <0.01 galactose...
17. How should the specific activity of an enzyme preparation change during its purification? A. Increase...
17. How should the specific activity of an enzyme preparation change during its purification? A. Increase B. Decrease C. Remain unchanged D. Unknown; it must be determined for each enzyme individually E. Enzymes cannot be purified 18 18. Which amino acid is being titrated in the figure to the right? A. D B.G C. H D. K E. R NOH The next question concerns the SDS-PAGE figure below. Lane 1 contains an unknown sample. Lane M contains a mixture of...
I only need help with part D. The answer is 0.065 sec-1, but I need help...
I only need help with part D. The answer is
0.065 sec-1, but I need help to understand why.
You are studying a dehydrogenase enzyme that catalyzes the formation of NADH from NAD^+. Subsequent studies determined that NADU will absorb at 340 nm while NAD^+ will not. The extinction coefficient for NADH is 6220 M^-1 cm^-1. The reaction volume is 750 mu L total and the substrate molecular weight is 223 g/mol. You used 20 mu L of enzyme from...
Catalase (MW250,000Da) has one of the highest turnover numbers we know of. It catalyzes the decomposition...
Catalase (MW250,000Da) has one of the highest turnover numbers we know of. It catalyzes the decomposition of hydrogen peroxide as shown in the reaction (2H2O2à2H2O + O2) If 15.0 mg of pure catalasecatalyzes the decomposition of 0.40 g of H2O2in 1 min at 37 °C at its Vmax, what is the turnover number (kcat) of catalase(in units of sec-1)? Plot a graph of Abs vs. time at different concentrations of enzyme and replot a figure and find out the optimal...
1. MICHAELIS-MENTON-(REQUIRED) a. Draw a simple graph, showing the classic Michaelis-Menton plot of enzyme activity as...
1. MICHAELIS-MENTON-(REQUIRED) a. Draw a simple graph, showing the classic Michaelis-Menton plot of enzyme activity as a function of substrate concentration; label both axes. Write the associated Michaelis-Menton equation and show the location of Km and Vmax on your graph. b. Draw a second graph showing the classic Lineweaver-Burk plot; label both axes. Show the location of Km and Vmax on your graph. Discuss which plot is the most useful to determine Vmax. Draw a second line on each graph...
1. (4 points) Given that the average molecular weight of an amino acid is 110 daltons...
1. (4 points) Given that the average molecular weight of an amino acid is 110 daltons (Da), determine the following. Remember to convert between daltons and kilodaltons (kDa) where necessary. Each of your answers must be numerically correct and expressed in proper units (1 pt) and show how the value was arrived at using the amino acid molecular weight above (1 pt) for you to receive full credit. A. A molecule of human a-tubulin has a molecular weight of 50.1...
Based on the document below, 1. Describe the hypothesis Chaudhuri et al ids attempting to evaluate;...
Based on the document below,
1. Describe the hypothesis Chaudhuri et al ids attempting to
evaluate; in other words, what is the goal of this paper? Why is he
writing it?
2. Does the data presented in the paper support the hypothesis
stated in the introduction? Explain.
3.According to Chaudhuri, what is the potential role of thew
alkaline phosphatase in the cleanup of industrial waste.
CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP 8.5, 9, 9.5, 10,...
Lab 8: Exercises EXERCISE 8.1 GM is a 20 y/o male who was admitted to the...
Lab 8: Exercises EXERCISE 8.1 GM is a 20 y/o male who was admitted to the ICU for severe closed head injury and multiple fractures resulting from a fighting engagement at the bar on his birthday. According to his friends, his Ht is 178 cm. His measured Wt at admission is 76 kg. His lab values were normal except for low Hgb and Hct due to bleeding (which was well controlled). He was unconscious, thus was tube fed for one...
1. According to the paper, what does lactate dehydrogenase (LDH) do and what does it allow...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
ARE MY ANSWERS CORRECT? 25 questions 1. what an A/R aging analysis is, its purpose, and...
ARE MY ANSWERS CORRECT? 25 questions 1. what an A/R aging analysis is, its purpose, and how it is created. Used to estimate amount needed in Allowance for Bad Debts Account (a contra account) A/R Days Outstanding 0-30 31-60 61-90 Over 90 Under each term list all A/Rs that are not paid by date Use historical experience to estimate the percentage of A/R for each date period to determine allowance for Bad Debts What the three major cost components are...
included proper units when HLLL 1. The following kinetic data were generated for the enzyme SpB1, this enzyme has an unknown biological function. Using the information provided below answer questions la-le. All reactions had 0.1 mg/mL SPBT which based on amino acid sequence and gel filtration column chromatography has a molecular weight of 42,500 Daltons. S002- Substrate 1 Substrate 2 KM (MM) kcat (sec) glucose >50 <0.01 glucose NADP 4+1 0.5+0.07 glucose NAD 2718 2 +0.05 galactose >50 <0.01 galactose...
17. How should the specific activity of an enzyme preparation change during its purification? A. Increase B. Decrease C. Remain unchanged D. Unknown; it must be determined for each enzyme individually E. Enzymes cannot be purified 18 18. Which amino acid is being titrated in the figure to the right? A. D B.G C. H D. K E. R NOH The next question concerns the SDS-PAGE figure below. Lane 1 contains an unknown sample. Lane M contains a mixture of...
I only need help with part D. The answer is
0.065 sec-1, but I need help to understand why.
You are studying a dehydrogenase enzyme that catalyzes the formation of NADH from NAD^+. Subsequent studies determined that NADU will absorb at 340 nm while NAD^+ will not. The extinction coefficient for NADH is 6220 M^-1 cm^-1. The reaction volume is 750 mu L total and the substrate molecular weight is 223 g/mol. You used 20 mu L of enzyme from...
1. MICHAELIS-MENTON-(REQUIRED) a. Draw a simple graph, showing the classic Michaelis-Menton plot of enzyme activity as a function of substrate concentration; label both axes. Write the associated Michaelis-Menton equation and show the location of Km and Vmax on your graph. b. Draw a second graph showing the classic Lineweaver-Burk plot; label both axes. Show the location of Km and Vmax on your graph. Discuss which plot is the most useful to determine Vmax. Draw a second line on each graph...
1. (4 points) Given that the average molecular weight of an amino acid is 110 daltons (Da), determine the following. Remember to convert between daltons and kilodaltons (kDa) where necessary. Each of your answers must be numerically correct and expressed in proper units (1 pt) and show how the value was arrived at using the amino acid molecular weight above (1 pt) for you to receive full credit. A. A molecule of human a-tubulin has a molecular weight of 50.1...
Based on the document below,
1. Describe the hypothesis Chaudhuri et al ids attempting to
evaluate; in other words, what is the goal of this paper? Why is he
writing it?
2. Does the data presented in the paper support the hypothesis
stated in the introduction? Explain.
3.According to Chaudhuri, what is the potential role of thew
alkaline phosphatase in the cleanup of industrial waste.
CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP 8.5, 9, 9.5, 10,...
Lab 8: Exercises EXERCISE 8.1 GM is a 20 y/o male who was admitted to the ICU for severe closed head injury and multiple fractures resulting from a fighting engagement at the bar on his birthday. According to his friends, his Ht is 178 cm. His measured Wt at admission is 76 kg. His lab values were normal except for low Hgb and Hct due to bleeding (which was well controlled). He was unconscious, thus was tube fed for one...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
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