explain how addition or removal of a phosphate group can be used to control the activity...
- Part A Why is covalent modification used to regulate the activity of an enzyme? Match the words in the left column to the appropriate blanks in the sentences on the right. Make certain each sent Reset Hel can be activated or deactivated as needed Covalent modification is useful to regulate the activity of enzymes because it is ; enzymes by addition or removal of a covalently bonded group (e.g., a phosphate group). irreversible can only be activated reversible can...
a. Which step in glycolysis is the major control point? Step 10, the addition of phosphate to phosphoenolpyruvate. Step 3, the addition of phosphate to fructose 6-phosphate. Step 1, the addition of phosphate to glucose. b. What compounds act as positive effectors of the enzyme that catalyzes this reaction? Citrate МАТР O Pyruvate ADP AMP c. What compounds are negative effectors? O AMP Citrate ADP ATP Glucose
Protein phosphatases catalyze removal of phosphate groups from proteins. How would the activity of a protein phosphatase affect a cell's response to growth factors? a) Decrease proliferation b) Decrease glucose production c) Increase proliferation d) Increase glucose production
The removal of a phosphate group is catalyized by? O Protein Phosphatase O None of the above ОАТР Antigens O Protein kinase
The activity of many enzymes is regulated by reversible covalent modifications. Kinases add a phosphate group from an ATP to a specific hydroxyl group on an amino acid residue within an enzyme. Which of the following amino acids can be phosphorylated by a kinase? (Select all that apply) Cys His Tyr Thr Phe Ser Val
The removal of a phosphate group is catalyized by? O Protein Phosphatase O None of the above ОАТР O Antigens O Protein kinase
Explain how stressing the system, Fe3+ + SCN1- ⇄ FeSCN2+, with the addition or removal of ions and temperature increased or decreased the concentration of the species. How did this affect the shift of the reaction in either the forward direction to produce more thiocyanato iron (III) ion or the reverse direction to remove thiocyanato iron (III) ion?
. ATP synthase activity is reversible and can be used to generate ADP and inorganic phosphate from ATP. TRUE FALSE
In a standard assay for LDH activity we used a phosphate buffer, pyruvate, and NADH with the LDH enzyme. I don't understand what happens when we replace the NADH with NADPH and why does LDH have a preference for NADH over NADPH
The effect of inorganic phosphate on the activity of the citric acid cycle can be studied in mitochondrial extracts by monitoring the rate of CO2 production. a. Why is the citric acid cycle dependent upon inorganic phosphate? b. Acetyl CoA is added to a rat liver mitochondrial extract containing excess NAD+, FAD, and GDP. The rate of CO2 production increases and then levels off very quickly; the addition of addition acetyl CoA has no effect. Explain why CO2 production begins...