Predict how the following environmental changes will affect the pKa of a K residue in a...
Predict how the following environmental changes will affect the pKa of a K residue in a protein:
• a) a D residue in the same protein is brought into close
proximity
• b) the K side-chain shifts from the outside of the protein to a
nonpolar site inside
• c) an R residue in the same protein is brought into close
proximity.
In each case, will the pKa go up, down, or remain the same?
Explain.
Homework Answers
a) pKa / K can be either +/0 in charge. Bringing a “D–” into close proximity favors K+. For K to have a positive charge more of the time, its pKa increases( higher pKa’s are better bases).
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