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why is it important to observe Effect 30 uM of Nocodazole has on hyphal Fungal Tip...

why is it important to observe Effect 30 uM of Nocodazole has on hyphal Fungal Tip Growth rate and nuclei positions Neurospora crassa(Fungi)?

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Nocodazole is a drug and an anti neoplastic agent have the ability to polymerise microtubules.

In 30 um nocodazole, the Neurospora crassa assemblies mitochondrial propagation, forward with elongation of hypha and split upon the apical branching. The nuclei of N. crassaa are located at a distance of approximately 20-30 mm from the growth point in fungal cell.

With the uses nocodazole, we can depolymerise the microtubule network and can be also reveals its region of orgin. So, it is important to observe the synchronisation of cell division cycle and arrangement of cellular organelles in fungal cells of N. crassa when 30 um nocodazole is treated .

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