The molecular weight of wheat germ acid phosphatase is 55,000. What is its turnover number under...
The molecular weight of wheat germ acid phosphatase is 55,000. What is its turnover number under our experimental conditions? Use Vmax from the uninhibited enzyme. (Recall that the enzyme stock was 0.15 mg/ml). Vmax = 6.04 x 10^-6
Homework Answers
Add Answer to:
The molecular weight of wheat germ acid phosphatase is 55,000.
What is its turnover number under...
Enzyme Kinetics - Farrell/Taylor 133 3. Enzyme X has a molecular weight of 48,000. It converts...
Enzyme Kinetics - Farrell/Taylor 133 3. Enzyme X has a molecular weight of 48,000. It converts substrate Z into product Y. Z absorbs at 340 nm, and Y absorbs at 480 nm. a. At what wavelength do you measure the change in absorbance to assay for enzyme X? Does the absorbance increase or decrease over time? b. If Vmax = 60 pmol/min and you use 400L of a 0.1 mg/mL solu- tion of enzyme, what is the turnover number? 4....
Can anyone help with parts D and E? Ibuprofen [a-methyl-4-(isobutyl)phenylacetic acid; Mr 206] reversibly inhibits cyclo-oxygenase...
Can anyone help with parts D and E?
Ibuprofen [a-methyl-4-(isobutyl)phenylacetic acid; Mr 206] reversibly inhibits cyclo-oxygenase (COX-2), a Michaelis enzyme that converts arachidonate to prostaglandin G2 (PPG2) thereby preventing the fever, inflammation and pain induced by prostaglandins. The following table shows a kinetic analysis of COX-2 in the absence and presence of 10 mg/ml Ibuprofen [Arachidonate] Vo mM/min w/o Ibuprofen Vo, mM/min w/ Ibuprofen mM 0.5 23.5 16.67 25.25 30.49 1.0 32.2 1.5 36.9 2.5 41.8 37.04 38.91 3.5 44.0...
Question to answer from the lab: What aspect(s) of the approach taken is/are NOT especially green?...
Question to answer from the lab: What aspect(s) of the approach taken is/are NOT especially green? What changes might be made to make the overall process greener? The Lab Experiment Summary Polyethylene glycols such as PEG-400 are currently of great interest to organic chemists, since their application as reaction solvents is very intriguing (1). PEG-400 is non-volatile and nonflammable in nature, and also possesses important properties such as recyclability, ease of work-up, thermal stability and economical cost. In addition, the...
Based on the document below, 1. Describe the hypothesis Chaudhuri et al ids attempting to evaluate;...
Based on the document below,
1. Describe the hypothesis Chaudhuri et al ids attempting to
evaluate; in other words, what is the goal of this paper? Why is he
writing it?
2. Does the data presented in the paper support the hypothesis
stated in the introduction? Explain.
3.According to Chaudhuri, what is the potential role of thew
alkaline phosphatase in the cleanup of industrial waste.
CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP 8.5, 9, 9.5, 10,...
help on number 3 here is lab we did EXPERIMENT 19 An Analysis of Commercial Antacids...
help on number 3 here is lab we did
EXPERIMENT 19 An Analysis of Commercial Antacids Rolaids Alka Seltzer OBJECTIVES: Determine the per gram neutralizing ability of a commercial antacio Determine the per gram neutralizing ability of sodium bicarbonate Compare the effectiveness of several antacids. INTRODUCTION: "How do you spell relief two hecimie The parietal cells in the stomach secrete sufficient hydrochloric aci 0.10 M The flow of HCl increases when food enters the stomach and so acid secrete sufficient...
1. According to the paper, what does lactate dehydrogenase (LDH) do and what does it allow...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
just one example/demonstration! Data needed to be calculated is in highlighted in green boxes. And I...
just one example/demonstration!
Data needed to be calculated is in highlighted in green boxes.
And I highlighted in red an equation (not sure if thats what you
use to calculate it) And ignore the lab instructions on completeing
a graph!! I already know how to do that in excel, just curious how
Ln (relative rate) and 1/T in K^-1 is calculated by hand*
here is the rest of that lab leading up to the question as I
know its typically...
Can you please explain in simple terms the methods of this experiment? I'm having difficulty visualizing...
Can you please explain in simple terms the methods of this experiment? I'm having difficulty visualizing and understanding what is being done. I have copied and pasted some of the text from the article. Thank you! Abstract : Obesity, high-fat diets, and subsequent type 2 diabetes (T2DM) are associated with cognitive impairment. Moreover, T2DM increases the risk of Alzheimer's disease (AD) and leads to abnormal elevation of brain beta-amyloid levels, one of the hallmarks of AD. The psychoactive alkaloid caffeine...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated sludge operation that adversely affect effluent quality with origins in the engineering, hydraulic and microbiological components of the process. The real "heart" of the activated sludge system is the development and maintenance of a mixed microbial culture (activated sludge) that treats wastewater and which can be managed. One definition of a wastewater treatment plant operator is a "bug farmer", one who controls the aeration...
1. Which of the following are the sites within the human body where carbon dioxide and...
1. Which of the following are the sites within the human body where carbon dioxide and oxygen are exchanged? A. Alveoli B. Arteries C. Synapses D. Venules 2. Which of the following describes the most important reason for repeating an experimental investigation? A. To verify the validity of the original findings B. To expand upon the original investigation C. To manipulate the independent variable D. To attempt to disprove the hypothesis 3. Lithium has an atomic number of 3 and...
Enzyme Kinetics - Farrell/Taylor 133 3. Enzyme X has a molecular weight of 48,000. It converts substrate Z into product Y. Z absorbs at 340 nm, and Y absorbs at 480 nm. a. At what wavelength do you measure the change in absorbance to assay for enzyme X? Does the absorbance increase or decrease over time? b. If Vmax = 60 pmol/min and you use 400L of a 0.1 mg/mL solu- tion of enzyme, what is the turnover number? 4....
Can anyone help with parts D and E?
Ibuprofen [a-methyl-4-(isobutyl)phenylacetic acid; Mr 206] reversibly inhibits cyclo-oxygenase (COX-2), a Michaelis enzyme that converts arachidonate to prostaglandin G2 (PPG2) thereby preventing the fever, inflammation and pain induced by prostaglandins. The following table shows a kinetic analysis of COX-2 in the absence and presence of 10 mg/ml Ibuprofen [Arachidonate] Vo mM/min w/o Ibuprofen Vo, mM/min w/ Ibuprofen mM 0.5 23.5 16.67 25.25 30.49 1.0 32.2 1.5 36.9 2.5 41.8 37.04 38.91 3.5 44.0...
Based on the document below,
1. Describe the hypothesis Chaudhuri et al ids attempting to
evaluate; in other words, what is the goal of this paper? Why is he
writing it?
2. Does the data presented in the paper support the hypothesis
stated in the introduction? Explain.
3.According to Chaudhuri, what is the potential role of thew
alkaline phosphatase in the cleanup of industrial waste.
CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP 8.5, 9, 9.5, 10,...
help on number 3 here is lab we did
EXPERIMENT 19 An Analysis of Commercial Antacids Rolaids Alka Seltzer OBJECTIVES: Determine the per gram neutralizing ability of a commercial antacio Determine the per gram neutralizing ability of sodium bicarbonate Compare the effectiveness of several antacids. INTRODUCTION: "How do you spell relief two hecimie The parietal cells in the stomach secrete sufficient hydrochloric aci 0.10 M The flow of HCl increases when food enters the stomach and so acid secrete sufficient...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
just one example/demonstration!
Data needed to be calculated is in highlighted in green boxes.
And I highlighted in red an equation (not sure if thats what you
use to calculate it) And ignore the lab instructions on completeing
a graph!! I already know how to do that in excel, just curious how
Ln (relative rate) and 1/T in K^-1 is calculated by hand*
here is the rest of that lab leading up to the question as I
know its typically...
Most questions answered within 3 hours.
-
A cohort study is conducted to determine whether smoking is
associated with an increased risk of...
asked 1 minute ago -
A statistics student finds herself struggling with a newspaper
article stating that only eighteen percent of...
asked 1 minute ago -
Create the pseudo-code/flowchart for an application class named
Monogram. Its main() method inputs three variables that...
asked 1 minute ago -
How many liters of water are required to dissolve 1.00 g of
silver chromate? Express your...
asked 4 minutes ago -
Hot: T_inlet = 80, T_out = 65
Cold: T_inlet = 10, T_out = 25
Explain in...
asked 4 minutes ago -
Two protons fly in different directions and collide. They both
have a total energy of 1.5...
asked 14 minutes ago -
What is the oxidation number of each atom in sodium phosphate,
Na3PO4?
>>> SHOW YOUR WORK...
asked 20 minutes ago -
D company purchased goods with a list price of $60000, subject
to trade discounts of 20%...
asked 22 minutes ago -
Transposable elements make up more than 40% of the human genome
and are inserted more-or-less randomly...
asked 33 minutes ago -
Can I get some samples of the following:
1) Write four sentences in first person, singular....
asked 38 minutes ago -
Described how search engines work. Be sure to describe in detail
.
asked 38 minutes ago -
The position of a particle as a function of time is given by
x=(2.0m/s)t+(−3.0m/s3)t^3. Part A...
asked 44 minutes ago