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There is a cell line that is used as a model to study skin cancer. It...

There is a cell line that is used as a model to study skin cancer. It is desired to identify disease markers at the protein level. If in addition to this tumor line, we also have healthy cells. Explain an experimental strategy, which, among other technologies, includes 2D electrophoresis and centrifugation, which allows you to generate proteins that serve as markers in the diagnosis of skin cancer.

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Experimental strategy has attached below

For use a biomarker of a protein , there must be a cut off value for that protein with high specificity and sensitivity. So that it could be used as a biomarker to diagnose a particular disease.

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