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Cyanide is a non-competitive inhibitor of Cytochrome c Oxidase. What Km would you expect if you...

Cyanide is a non-competitive inhibitor of Cytochrome c Oxidase. What Km would you expect if you treated 4µM cytochrome oxidase with enough cyanide to lower the enzymes Vmax to 10 units of activity?

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Answer #1

In case of Non-competitive inhibition, the inhibitor binds to the enzyme or enzyme substrate complex and reduces the activity of the enzyme. Vmax is changed (reduced) in case of non competitive inhibition while Km remains same.

Since Cyanide is a non-competitive inhibitor of Cytochrome c Oxidase so Km will remain unchanged.

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