1.Why does the separation of the different components occur within the column?

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1.Why does the separation of the different components occur within the column?

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Answer 1

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Ans: 1. Column chromatography is based on adsorption of compounds on adsorbent (silica).

2. Different compounds are having different polarity so they will adsorb differently through hydrogen bonding to silica used in column.

3. If compound is more polar it will get adsorbed mor tightly and compounds which are non polar which will travel with solvent fastly.

4. So if compound with more polar functionality travel through coloumn slowly and compounds which are non polar travel fastly with solvent.

In this way different compounds will get separated in column based on polarity of components.

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