Question 1: (column chromatography) a. What effect on the chromatographic separation is expected ...
Question 1: (column chromatography)
a. What effect on the chromatographic separation is expected if solvent level is below the adsorbent?
b. Once the chromatographic column was prepared, why was it important to allow the level of the liquid in the column to drop to the level of the stationary phase before applying the solution of the compounds to be separated?
c. Name two methods of carrying out the column chromatography technique?
d. What are the two methods of packing a chromatographic column?
Homework Answers
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Question 1: (column chromatography) a. What effect on the chromatographic separation is expected ...
What effect will the following factors have on a column chromatographic separation with alumina as the...
What effect will the following factors have on a column chromatographic separation with alumina as the stationary phase? A) methanol is used as the initial eluting solvent B) The level of the eluting solvent falls below the top of the column? C) Heptane is used as the initial eluting solvent
What effect will the following factors have on a chromatographic separation: too strong an adsorbent; a...
What effect will the following factors have on a chromatographic separation: too strong an adsorbent; a collection of large elution fractions; solvent level below the top of column adsorbent?
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is...
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is based on analytes being partitioned between a stationary phase (not moving) and a mobile phase (moving). What is most commonly used as stationary phase in a column? Is this material polar or non-polar? In this experiment, you will use different solvents to run column chromatography separation of different pigments extracted from spinach leaves. These solvents are a mixture of acetone and hexanes. (a) What...
1. What is the purpose of column chromatography 2. What is the stationary phase? Mobile phase?...
1. What is the purpose of column chromatography 2. What is the stationary phase? Mobile phase? 3. What is the purpose of the cotton (glass wool) in column chromatography? 4. What is the purpose of the sand? 5. Why is it important to prevent the column from drying? 6. What can happen if you disrupt your even layer of silica gel? 7. Which compounds come off the column first, polar or nonpolar? 8. Keyon synthesized acetylferrocene (orange) in organic chemistry...
Question 1 Which of the following is the advantage of straight packed column? Selected Answer: It...
Question 1 Which of the following is the advantage of straight packed column? Selected Answer: It can be repacked easily Answers It can be packed uniformly It can be repacked easily It is compact It is easier to heat it evenly Question 2 Which of the following statements most accurately describes thin-layer chromatography? Selected Answer: technique used to separate non-volatile mixtures, the mobile phase has different properties from the stationary phase. Answers The mobile phase is the solvent also called...
1. What are the main differences between thin layer chromatography (TLC) and column chromatography (CC)? 2. Are CC...
1. What are the main differences between thin layer chromatography (TLC) and column chromatography (CC)? 2. Are CC and TLC operations conducted concurrently? Explain. 3. Name a few packing materials used in packing columns in Column Chromatography operations. 4. Explain the role of eluting solvent(s) in column chromatography procedures. 5. When performing column chromatography, which of the following compounds would you expect to elute first: Ferrocene of acetylferrocene? Explain why.
8.5 TLC is commonly employed with column chromatography to monitor the separation of colorless compounds. The...
8.5 TLC is commonly employed with column chromatography to monitor the separation of colorless compounds. The column is allowed to elute into many containers, usually test tubes. This is followed by a TLC on a long plate containing the same stationary phase as the column chromatography. Since a large number of tubes must be spotted, by spotting only every other (or every three etc.) tube on the plate. After visualization (by UV), the TLC plate is used to judge which...
Question 1 (1 point) Saved Chromatography is a technique that separates substances in a mixture based...
Question 1 (1 point) Saved Chromatography is a technique that separates substances in a mixture based on the interactions of the mixture components with a mobile phase and a stationary phase. True False Question 2 (1 point) Saved The mobile phase carries substances along at a rate that is dependent on the attraction of the substance to the stationary phase. True False Question 3 (1 point) Use 10 mL of 10% isopropanol followed by 10 mL of distilled water to...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography?...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
Table 2: Alcohol and Ketone Standards GC Retention Times GC#: Retention time Alcohol Standard Mixture Phent...
Table 2: Alcohol and Ketone Standards GC Retention Times GC#: Retention time Alcohol Standard Mixture Phent ion= 2.05Smin low Peak 1 Compound Name: athanol Rapesnal utanal aclapeatanal 4.755min oilng 6.895min .230 min Peak 2 Compound Name: Peak 3 Compound Name: Peak 4 Compound Name: GC#:1 Ketone Standard Mixture Retention time 2.575un Peak 1 Compound Name: Acclono Butanon a Pentanone 2tHex anon Peak 2 Compound Name: min Peak 3 Compound Name: 615 min 1D. 307 min Peak 4 Compound Name: Part...
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is based on analytes being partitioned between a stationary phase (not moving) and a mobile phase (moving). What is most commonly used as stationary phase in a column? Is this material polar or non-polar? In this experiment, you will use different solvents to run column chromatography separation of different pigments extracted from spinach leaves. These solvents are a mixture of acetone and hexanes. (a) What...
1. What is the purpose of column chromatography 2. What is the stationary phase? Mobile phase? 3. What is the purpose of the cotton (glass wool) in column chromatography? 4. What is the purpose of the sand? 5. Why is it important to prevent the column from drying? 6. What can happen if you disrupt your even layer of silica gel? 7. Which compounds come off the column first, polar or nonpolar? 8. Keyon synthesized acetylferrocene (orange) in organic chemistry...
Question 1 Which of the following is the advantage of straight packed column? Selected Answer: It can be repacked easily Answers It can be packed uniformly It can be repacked easily It is compact It is easier to heat it evenly Question 2 Which of the following statements most accurately describes thin-layer chromatography? Selected Answer: technique used to separate non-volatile mixtures, the mobile phase has different properties from the stationary phase. Answers The mobile phase is the solvent also called...
1. What are the main differences between thin layer chromatography (TLC) and column chromatography (CC)? 2. Are CC and TLC operations conducted concurrently? Explain. 3. Name a few packing materials used in packing columns in Column Chromatography operations. 4. Explain the role of eluting solvent(s) in column chromatography procedures. 5. When performing column chromatography, which of the following compounds would you expect to elute first: Ferrocene of acetylferrocene? Explain why.
8.5 TLC is commonly employed with column chromatography to monitor the separation of colorless compounds. The column is allowed to elute into many containers, usually test tubes. This is followed by a TLC on a long plate containing the same stationary phase as the column chromatography. Since a large number of tubes must be spotted, by spotting only every other (or every three etc.) tube on the plate. After visualization (by UV), the TLC plate is used to judge which...
Question 1 (1 point) Saved Chromatography is a technique that separates substances in a mixture based on the interactions of the mixture components with a mobile phase and a stationary phase. True False Question 2 (1 point) Saved The mobile phase carries substances along at a rate that is dependent on the attraction of the substance to the stationary phase. True False Question 3 (1 point) Use 10 mL of 10% isopropanol followed by 10 mL of distilled water to...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
Table 2: Alcohol and Ketone Standards GC Retention Times GC#: Retention time Alcohol Standard Mixture Phent ion= 2.05Smin low Peak 1 Compound Name: athanol Rapesnal utanal aclapeatanal 4.755min oilng 6.895min .230 min Peak 2 Compound Name: Peak 3 Compound Name: Peak 4 Compound Name: GC#:1 Ketone Standard Mixture Retention time 2.575un Peak 1 Compound Name: Acclono Butanon a Pentanone 2tHex anon Peak 2 Compound Name: min Peak 3 Compound Name: 615 min 1D. 307 min Peak 4 Compound Name: Part...
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