What effect will the following factors have on a chromatographic separation: too strong an adsorbent; a...
What effect will the following factors have on a chromatographic separation:
too strong an adsorbent;
a collection of large elution fractions;
solvent level below the top of column adsorbent?
Homework Answers
A) If too strong adsorbent will be used then in that case the eluent might not seperate the compounds easily.
B) collection of excess or large elution fractions might create problem by which we probably get two compounds into the same fraction.
C) If the solvent level will be below the adsorbent then it might possible that the column will be dry.
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What effect will the following factors have on a chromatographic
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too strong an adsorbent;
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Question 1: (column chromatography) a. What effect on the chromatographic separation is expected ...
Question 1: (column chromatography) a. What effect on the chromatographic separation is expected if solvent level is below the adsorbent? b. Once the chromatographic column was prepared, why was it important to allow the level of the liquid in the column to drop to the level of the stationary phase before applying the solution of the compounds to be separated? c. Name two methods of carrying out the column chromatography technique? d. What are the two methods of packing a...
Hi can someone help me with my pre lab theory questions (1,2,3) on rate of elution...
Hi can someone help me with my pre lab theory questions (1,2,3)
on rate of elution or Rf? Here is some background below. Please
help explain as many as possible (1-3)! I dont really get the
relation ships in this lab, thank you!
1. What factors affect the rate of elution in organic compounds? 2. Explain what is the relationship between polarities of compounds (polar/non- polar compound) and rate of elution (Rf). 3. Explain what is the relationship between solvent...
The answers are not "Both factors have an effect" Answer the question based on the following...
The answers are not "Both factors have an
effect"
Answer the question based on the following interaction plot: 5 Factor B Level 1 Level 2 4 3 Cell Mean 2. 1 0 1 2 3 Factor A Neither factor looks to have an effect Only Factor A has an effect Only Factor B has an effect Both factors have an effect There is an interaction between the factors [1 pt(s)] Submit Answer Incorrect. Tries 1/2 Previous Tries Answer the question...
what is the prediction of the unknown amino acids mixture based on the strip from thin layer chromatography sheet? The unknown mixtures can be ala plus asp, ala plus asp plus lys, lys plus asp or ala...
what is the prediction of the unknown amino acids mixture
based on the strip from thin layer chromatography sheet?
The unknown mixtures can be ala plus asp, ala plus asp plus
lys, lys plus asp or ala plus lys.
anion exchange resin was used
Tubes 2,3, 6 and 7 had a spot appear that was light pink
We were unable to transcribe this imageWe were unable to transcribe this imageH-) to the column followed by a sufficient amount of the...
a) what effect does the change in internuclear separation in a diatomic molecule due to its vibration (the binding energ...
a) what effect does the change in internuclear separation in a diatomic molecule due to its vibration (the binding energy curve is asymmetric) have on the rotational energy levels of molecule? b)Explain why the separation between vibrational levels is somewhat smaller in an excited electronic state than in the ground electronic state. Explain the same effect for rotational states. c)show the ratio number of molecules in rotational level r to the number in the r=0 level, in a sample at...
help! 14. Which of the following factors does effect on DNA fragments separation during electrophoresis a....
help!
14. Which of the following factors does effect on DNA fragments separation during electrophoresis a. Size b. Electrical charge c. Nucleotides sequences d. All of the above Match the following with q 15-18 a. A necessary cofactor for DNA polymerization b. To break cell membrane open c. Confirm plant-based sample d. Detection of GMO e. Cell membrane lysis 15. Salt solution and heat 16. Primer of CaMw promoter 17. Primer of Nopalin Synthetase virus terminator 18. Magnesium chloride 19-...
2-4 What effect do you think each of the following factors should have on the interest...
2-4 What effect do you think each of the following factors should have on the interest rate that a firm must pay on a new issue of long-term dele? Indicate whether each factor would tend to raise, lower, or have an indeterminate effect on the interest tate, and then explain why. a. The firm uses bonds rather than a term loun. b. The finnuses debentures rather than first mortgage bonds. c. The firm makes its bonds convertible into common stock....
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help needed with this problem set Refer to the HPLC chromatograms below for a 5-component mixture of organic acids for the following questions. The specific HPLC parameters for chromatogram A (...
help needed with this problem set
Refer to the HPLC chromatograms below for a 5-component mixture of organic acids for the following questions. The specific HPLC parameters for chromatogram A (top) are described in the figure title and the insert. You only need the lecture notes and/or your personal experience to answer those questions. Mobile phase: 30 % MeCN, 70 % TFA, 0.1 M (pH 2.0) low rate: 1.0 mL/min 2Benzok Acid Maleic Aci 1. (a) Which component has the...
Hi can someone help me with my pre lab theory questions (1,2,3)
on rate of elution or Rf? Here is some background below. Please
help explain as many as possible (1-3)! I dont really get the
relation ships in this lab, thank you!
1. What factors affect the rate of elution in organic compounds? 2. Explain what is the relationship between polarities of compounds (polar/non- polar compound) and rate of elution (Rf). 3. Explain what is the relationship between solvent...
The answers are not "Both factors have an
effect"
Answer the question based on the following interaction plot: 5 Factor B Level 1 Level 2 4 3 Cell Mean 2. 1 0 1 2 3 Factor A Neither factor looks to have an effect Only Factor A has an effect Only Factor B has an effect Both factors have an effect There is an interaction between the factors [1 pt(s)] Submit Answer Incorrect. Tries 1/2 Previous Tries Answer the question...
what is the prediction of the unknown amino acids mixture
based on the strip from thin layer chromatography sheet?
The unknown mixtures can be ala plus asp, ala plus asp plus
lys, lys plus asp or ala plus lys.
anion exchange resin was used
Tubes 2,3, 6 and 7 had a spot appear that was light pink
We were unable to transcribe this imageWe were unable to transcribe this imageH-) to the column followed by a sufficient amount of the...
help!
14. Which of the following factors does effect on DNA fragments separation during electrophoresis a. Size b. Electrical charge c. Nucleotides sequences d. All of the above Match the following with q 15-18 a. A necessary cofactor for DNA polymerization b. To break cell membrane open c. Confirm plant-based sample d. Detection of GMO e. Cell membrane lysis 15. Salt solution and heat 16. Primer of CaMw promoter 17. Primer of Nopalin Synthetase virus terminator 18. Magnesium chloride 19-...
2-4 What effect do you think each of the following factors should have on the interest rate that a firm must pay on a new issue of long-term dele? Indicate whether each factor would tend to raise, lower, or have an indeterminate effect on the interest tate, and then explain why. a. The firm uses bonds rather than a term loun. b. The finnuses debentures rather than first mortgage bonds. c. The firm makes its bonds convertible into common stock....
help needed with this problem set
Refer to the HPLC chromatograms below for a 5-component mixture of organic acids for the following questions. The specific HPLC parameters for chromatogram A (top) are described in the figure title and the insert. You only need the lecture notes and/or your personal experience to answer those questions. Mobile phase: 30 % MeCN, 70 % TFA, 0.1 M (pH 2.0) low rate: 1.0 mL/min 2Benzok Acid Maleic Aci 1. (a) Which component has the...
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