Explain why burst kinetics are observed for one chromogenic substrate but not another.
Chromogenic substrate are synthetically made and are designed to be highly selective as the natural substrate for that particular enzyme. And it follows single substrate mechanism in which that enzyme binds only one substrate. We know that, burst kinetics is a form of enzyme kinetics therefore burst kinetics are observed for one chromogenic substrate but not another due to chromogenic substrate's highly selective nature.
Explain why burst kinetics are observed for one chromogenic substrate but not another.
What kind of kinetics is observed in an enzymatic reaction, under conditions where the substrate concentration is much higher with respect to KM ([S] >> KM)? Assume that a monomeric and non-allosteric enzyme is considered. A. Cooperative B. First Order C. Zero Order D. The systerm is at equilibrium and no reaction occures E. Second Order
What is a burst phase? (Drawing a graph here would be helpful). Explain how the detection of a burst-phase in the short-timescale kinetics of the chymotrypsin enzymatic reaction helped researchers elucidate the mechanism of this enzyme reaction cycle?
In 50 words or more explain how chemical kinetics and chemical equilibrium are related to one another. Give a real world example of chemical kinetics.
Answer the following questions related to burst time prediction. A. Discuss why it is important to know the length of the next job or CPU burst and which scheduling algorithm requires future knowledge. B. Estimate the burst times under the following conditions: Tau = 12 Alpha=.25 Actual observed burst times are: 10, 15, 13, 10 C. Estimate the burst times under the following conditions: Tau = 12 Alpha=.75 Actual observed burst times are: 10, 15, 13, 10 D. What is...
. 52. What is a burst phase? (Drawing a graph here would be helpful). Explain how the detection of a burst-phase in the short-timescale kinetics of the chymotrypsin enzymatic reaction helped researchers elucidate the mechanism of this enzyme reaction cycle? 51. Draw a michaelis-menten graph (at right) and a linweaver burke (double-reciprocal) plot (at left) of an uninhibited enzymatic reaction. Be sure to label the axes with appropriate labels. In each of these TWO graphs, using the uninhibited curves as...
If the substrate concentration is varied with a fixed concentration of enzyme, it is observed that at low substrate concentrations that overall order while at high substrate concentrations, the reaction will be overall order. Select one: a. first; second b. first; third order c. first; zero d. second; second e. second; pseudo first order
b. Look at the graph below of how a competitive inhibitor affects the kinetics of an enzyme C. Rate of reaction is the Vmax of the enzyme affected? Why or why not: explain in terms of substrate concentration and enzyme active site saturation) Without inhibitor With competitive inhibitor d. is Vmax/2 affected? Why or why not: explain in terms of Vmax. Substrate concentration e. Is Km affected? Explain in terms of the active site. Hint a competitive inhibitor is competing...
24. Explain the energetic benefits of making the enzyme active state rather than to the substrate conformation. Also explain how contribute to enzyme action. In other words, how do these weak intera enzyme and the transition state lower the activation energy?) of making the enzyme active site complementary to the transition nation. Also explain how multiple weak interactions words, how do these weak interactions between the 25. 1/F. The pk's of titratable groups of amino acids remain constant irrespective of...
7. a) In an enzyme catalyzed reaction which follows the Michaelis-Menten kinetics. The substrate concentration (Km, Michaelis constant) needed to reach 50% of the maximum reaction velocity (Vmax) is 20 μΜ. What substrate concentration is required to obtain at least 75% of the maximum reaction velocity? Show the work to get full points. (5 points) b) You want to load 10 μg of protein in 15 μL into one of the 10% polyacrylamide gel well. The protein needs to be...
Explain why cell-substrate interactions matter in terms of biomaterial choice?