Question

. 52. What is a burst phase? (Drawing a graph here would be helpful). Explain how the detection of a burst-phase in the short-timescale kinetics of the chymotrypsin enzymatic reaction helped researchers elucidate the mechanism of this enzyme reaction cycle?

51. Draw a michaelis-menten graph (at right) and a linweaver burke (double-reciprocal) plot (at left) of an uninhibited enzymatic reaction. Be sure to label the axes with appropriate labels. In each of these TWO graphs, using the uninhibited curves as a reference add curves illustrating the data one would obtain for each of the types of reversible inhibition we discussed in class .

Answer 1

Answer:

51):

When molecules or ion bind to the enzyme via weak bonds & can dissociate auickly & effortlessly following the reaction this" is known as reversibile inhibition. There are three types of reversible inhibition: competitive inhibition! Here, &max does not change with respect to un inhibition. km increases & lowers the enzymer affinity for the substrate @ Now.competitive inhibition! Here Vmax reduced with respect to minhibition kom doesn't change, which means affinity of enzyme to substrate is not reduced. 3 un competitive inhibition Vmax as well as km de crearess. michaelis - xmenten eanation! Vo = max [6] Vo = [s]+ km kom w Line weaver - Burk equation: tomax T & Vrax where Vo= Initial velocity on the reaction. Vmax maximum velocity of the reaction. o substrate concentration. Kama Michaelis hententen contant

t'e won.competitive mhibitow Vmax msame competitive while max same, kinh um competitive No inhibition . Vmax inhibitor Vmaxs, km 1 -.. R competi max / e No inhibition / Uncompetive mant, kind Non competits tive im Vmaxl & om same Mr/vmax /km / Line weaner burk plot km 8 Michaelis menten flot [+ km is the Cs] when vo= ½Vmax]

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