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You cut the pBad-mTagBFP2 plasmid with the BamHI restriction enzyme only. There is only one BamHI...

You cut the pBad-mTagBFP2 plasmid with the BamHI restriction enzyme only. There is only one BamHI RE site. How many bands do you expect to see after you run the product on a gel? Would you expect the same result if you cut a linear PCR product that also only has one cut site? Explain your reasoning

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Given that the plasmid is cut using a BamHI restriction enzyme. A plasmid is generally small circular DNA molecule. Restriction enzymes cut the DNA molecules by recognizing specific sites called recognition sites. Here in this case we have only one restriction endonuclease site which can be recognized and cut by the BamHI restriction endonuclease. As BamHI recognized and cut a circular DNA molecule, now it becomes only one single linear molecule. When this product runs on a gel, the entire linear molecule moves as a single molecule and hence produces a single band in the gel.

If we cut a linear PCR product having one cut site, the enzyme cuts the linear molecule into two fragments. These two fragments move as two bands in the gel. Hence we can expect two bands with a linear molecule having a single cut site cut by an enzyme.

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