Question

question number 7

purifying plasmid from your cells, you will set up a teaction to cut the plasmid DNA with the restriction enzyme Bgl Il. See the map below for the positions of the Bgl Il restriction enzyme sites on the plasmid. How many DNA fragments would you expect if your restriction enzyme digest was complete and your plasmid contains insert? Question 7 Not yet graded/ 1 pts After you electrophoresed the plasmid digest in Question 6, your gel showed more bands than expected. Name 1 possible reason for the extra bands.

Answer 1

1. During electrophoresis, it will be obvious to get more bands if our insert lies within the Bgl II restriction site. if we digest the plasmid with BgI II, we can get two fragments only when the insert lies in any other restriction site. However if the insert kies in the restriction site, the 3 fragments will be produced as there are two Bgl II restriction sites which will produce 2 framents and the Bgl II site where insertion is present will also yield the insert as a separate fragement beacuse insertion of DNA fragment at particular restriction site also leads to creation of the same restriction sites at the ends of the insert.