Homework Answers
ANS1 - 2
ANS46 - Cas9 loads with a sgRNA sequence complementary to about 20 nucleotide sin the DNA target sequence,and this is basically where Cas9 will cut the DNA.
ANS39 - DNA with more of a negative charge is drawn more quickly towards the anode(the positively charged electrode).
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The picture above represents an agarose gel that was used to analyze plasmid DNA after it...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
RESTRICTION DIGEST ANALYSIS QUESTIONS(true or yes = A: false or no = b) 1. Larger DNA...
RESTRICTION DIGEST ANALYSIS QUESTIONS(true or yes = A: false or no = b) 1. Larger DNA fragments appear near the bottom of the gel. 2. Larger DNA fragments move more rapidly through the gel. D ONA that has many restriction sites for a certain endonuclease will be cut into more fragmets than DNA with fewer restriction sites. 4. Cutting DNA with many different endonucleases will result in more DNA fragments. 5. Restriction enzymes all recognize the same base sequence when...
Write true or false ______ 1. The DNA sequence of one human being is on average...
Write true or false ______ 1. The DNA sequence of one human being is on average 99.9% identical to another random human being. ______ 2. As of 2009, all living human beings have had their entire genome sequenced. ______ 3. The nucleotide bases present in a DNA sequence are A, U, G, C. ______ 4. Techniques that enabled scientists to clone genes were developed in the 1970s. ______ 5. A restriction enzyme is useful because it is a generic enzyme...
can someone explain throughly on how to find a-c??? thanks!!! The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel be...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
question number 7 purifying plasmid from your cells, you will set up a teaction to cut...
question number 7
purifying plasmid from your cells, you will set up a teaction to cut the plasmid DNA with the restriction enzyme Bgl Il. See the map below for the positions of the Bgl Il restriction enzyme sites on the plasmid. How many DNA fragments would you expect if your restriction enzyme digest was complete and your plasmid contains insert? Question 7 Not yet graded/ 1 pts After you electrophoresed the plasmid digest in Question 6, your gel showed...
2. Here are some DNA fragments that have been isolated by gel electrophoresis after being cut...
2. Here are some DNA fragments that have been isolated by gel electrophoresis after being cut with restriction enzynes. A. 5 '-ACTGACATAGGCACCCCTTAA-3 3'-TGACTGTATCCGTGGGG-5 5 '-TGACTGTATCCGTGGGG-3' 3 '-ACTGACATAGGCACCCCTTAA-5' 5 '-GGCATACTAGATCCACGTTAA-3 3'-CCGTATGATCTAGGTGC-5 5 '-GGCATACTAGATCCACGAATT-3 3'-CCGTATGATCTAGGTGC-5 E. 5 '-GGCATACTAGATCCACGGATC-3 3'-CCGTATGATCTAGGTGC-5 a. Which pair of these fragments has appropriate complementary sticky ends to get joined together in a recombinant DNA molecule? b. What enzyme would we use to join up the DNA backbones to make the make the recombinant molecule?
7.. In the controlled termination method of DNA sequencing, reading the gel from _____ gives the...
7.. In the controlled termination method of DNA sequencing, reading the gel from _____ gives the sequence in the _____ direction; _____ fragments that were terminated _____ in polymerization move faster down the gel a. bottom to top; 5′ to 3′; shorter; early b. top to bottom; 5′ to 3′; longer; early c. bottom to top; 5′ to 3′; longer; later d. top to bottom; 5′ to 3′; shorter; early e. bottom to top; 3′ to 5′; shorter; early 8....
A plasmid used as a cloning vector in E. coli must have… Does sequence similarity between...
A plasmid used as a cloning vector in E. coli must have… Does sequence similarity between genes play an important role in assigning gene function? Successful insertion of a DNA fragment into the multi-cloning region (restriction sites) of a recombinant plasmid is detected by what changes? Understand the concept of (restriction enzyme produced) DNA fragment separation by gel electrophoresis. In addition to restriction enzymes, which enzyme(s) are required to insert a fragment of DNA into a cloning vector? What is...
Use the following depiction of a gel of Hind III fragments to answer the questions: 1...
Use the following depiction of a gel of Hind III fragments to answer the questions: 1 Kb Ladder Possible Hind III Fragments: 1 Kb Ladder Sizes 10,000 8,000 564 bp 8,888 2.0 kb 2.3 kb 11 4.36 kb 4,000 3,000 2,500 2,000 1,500 6.5 kb 9.4 kb 23 kb 1,000 750 500 250 1. Based on the gel results, list the sizes of the chromosome fragments cloned: 2. Based on the gel results, list the sizes of the chromosome fragments...
In your previous prac session you digested your POTC-A plasmid DNA with 3 enzyme mixes (AB...
In your previous prac session you digested your POTC-A plasmid DNA with 3 enzyme mixes (AB and C). One mix contained the restriction endonuclease Kpnl alone, another contained both del and Not and another contained Sphi and Nhe, but you don't know which was which yet. The sites where these enzymes cut POTC-A are indicated on the plasmid map at the top of the page. Calculate the sizes of the DNA fragments that you would expect to see on your...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
RESTRICTION DIGEST ANALYSIS QUESTIONS(true or yes = A: false or no = b) 1. Larger DNA fragments appear near the bottom of the gel. 2. Larger DNA fragments move more rapidly through the gel. D ONA that has many restriction sites for a certain endonuclease will be cut into more fragmets than DNA with fewer restriction sites. 4. Cutting DNA with many different endonucleases will result in more DNA fragments. 5. Restriction enzymes all recognize the same base sequence when...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
question number 7
purifying plasmid from your cells, you will set up a teaction to cut the plasmid DNA with the restriction enzyme Bgl Il. See the map below for the positions of the Bgl Il restriction enzyme sites on the plasmid. How many DNA fragments would you expect if your restriction enzyme digest was complete and your plasmid contains insert? Question 7 Not yet graded/ 1 pts After you electrophoresed the plasmid digest in Question 6, your gel showed...
2. Here are some DNA fragments that have been isolated by gel electrophoresis after being cut with restriction enzynes. A. 5 '-ACTGACATAGGCACCCCTTAA-3 3'-TGACTGTATCCGTGGGG-5 5 '-TGACTGTATCCGTGGGG-3' 3 '-ACTGACATAGGCACCCCTTAA-5' 5 '-GGCATACTAGATCCACGTTAA-3 3'-CCGTATGATCTAGGTGC-5 5 '-GGCATACTAGATCCACGAATT-3 3'-CCGTATGATCTAGGTGC-5 E. 5 '-GGCATACTAGATCCACGGATC-3 3'-CCGTATGATCTAGGTGC-5 a. Which pair of these fragments has appropriate complementary sticky ends to get joined together in a recombinant DNA molecule? b. What enzyme would we use to join up the DNA backbones to make the make the recombinant molecule?
Use the following depiction of a gel of Hind III fragments to answer the questions: 1 Kb Ladder Possible Hind III Fragments: 1 Kb Ladder Sizes 10,000 8,000 564 bp 8,888 2.0 kb 2.3 kb 11 4.36 kb 4,000 3,000 2,500 2,000 1,500 6.5 kb 9.4 kb 23 kb 1,000 750 500 250 1. Based on the gel results, list the sizes of the chromosome fragments cloned: 2. Based on the gel results, list the sizes of the chromosome fragments...
In your previous prac session you digested your POTC-A plasmid DNA with 3 enzyme mixes (AB and C). One mix contained the restriction endonuclease Kpnl alone, another contained both del and Not and another contained Sphi and Nhe, but you don't know which was which yet. The sites where these enzymes cut POTC-A are indicated on the plasmid map at the top of the page. Calculate the sizes of the DNA fragments that you would expect to see on your...
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